文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Ravin R, Spira M E, Parnas H, Parnas I

Otto Loewi Centre for Cellular and Molecular Neurobiology, Department of Neurobiology, Hebrew University, Jerusalem, Israel.

J Physiol. 1997 Jun 1;501 ( Pt 2)(Pt 2):251-62. doi: 10.1111/j.1469-7793.1997.tb00001.x.

A technique has been developed to monitor neurotransmitter release simultaneously with intracellular Ca2+ concentration ([Ca2+]i) in single release boutons whose diameters range from 3 to 5 microns. 2. Using this technique, we have found a highly non-linear relationship between the rate of asynchronous release and [Ca2+]i. The Hill coefficient lies between 3 and 4. 3. The affinity (Kd) of the putative release-related Ca2+ receptor for asynchronous release was calculated to be in the range of 2-4 microM. 4. The same range of values of Hill coefficient and Kd were obtained when [Ca2+]i was elevated both by bath application of ionomycin and by repetitive stimulation at high frequency. 5. Our results show that the Ca2+ receptor(s) associated with asynchronous release exhibits high affinity for Ca2+.

已开发出一种技术，可在直径为3至5微米的单个释放小体中同时监测神经递质释放与细胞内钙离子浓度（[Ca2+]i）。2. 使用该技术，我们发现异步释放速率与[Ca2+]i之间存在高度非线性关系。希尔系数在3至4之间。3. 推测与释放相关的钙离子受体对异步释放的亲和力（Kd）经计算在2 - 4微摩尔范围内。4. 通过浴加离子霉素提高[Ca2+]i以及通过高频重复刺激提高[Ca2+]i时，均获得了相同范围的希尔系数和Kd值。5. 我们的结果表明，与异步释放相关的钙离子受体对钙离子具有高亲和力。

Ravin R, Spira M E, Parnas H, Parnas I

Otto Loewi Centre for Cellular and Molecular Neurobiology, Department of Neurobiology, Hebrew University, Jerusalem, Israel.

J Physiol. 1997 Jun 1;501 ( Pt 2)(Pt 2):251-62. doi: 10.1111/j.1469-7793.1997.tb00001.x.

A technique has been developed to monitor neurotransmitter release simultaneously with intracellular Ca2+ concentration ([Ca2+]i) in single release boutons whose diameters range from 3 to 5 microns. 2. Using this technique, we have found a highly non-linear relationship between the rate of asynchronous release and [Ca2+]i. The Hill coefficient lies between 3 and 4. 3. The affinity (Kd) of the putative release-related Ca2+ receptor for asynchronous release was calculated to be in the range of 2-4 microM. 4. The same range of values of Hill coefficient and Kd were obtained when [Ca2+]i was elevated both by bath application of ionomycin and by repetitive stimulation at high frequency. 5. Our results show that the Ca2+ receptor(s) associated with asynchronous release exhibits high affinity for Ca2+.

已开发出一种技术，可在直径为3至5微米的单个释放小体中同时监测神经递质释放与细胞内钙离子浓度（[Ca2+]i）。2. 使用该技术，我们发现异步释放速率与[Ca2+]i之间存在高度非线性关系。希尔系数在3至4之间。3. 推测与释放相关的钙离子受体对异步释放的亲和力（Kd）经计算在2 - 4微摩尔范围内。4. 通过浴加离子霉素提高[Ca2+]i以及通过高频重复刺激提高[Ca2+]i时，均获得了相同范围的希尔系数和Kd值。5. 我们的结果表明，与异步释放相关的钙离子受体对钙离子具有高亲和力。