Llinás R, Sugimori M, Silver R B
Department of Physiology and Neuroscience, New York University Medical Center, NY 10016, USA.
J Physiol Paris. 1995;89(2):77-81. doi: 10.1016/0928-4257(96)80554-7.
The time course for the calcium entry that triggers release was studied at the squid giant synapse by imaging light emission from n-aequorin-J intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per second. The results indicate that the calcium entry triggered by action potentials reaches a peak within 200 microseconds and has an overall duration of close to 800 microseconds, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions.
通过对注入突触前终末内的水母发光蛋白-J发出的光进行成像，在乌贼巨大突触处研究了触发递质释放的钙内流的时间进程。该成像利用了一个能够每秒采集4000帧图像的视频系统。结果表明，动作电位触发的钙内流在200微秒内达到峰值，总持续时间接近800微秒，这与在类似条件下通过电压钳制结果测定的突触前钙电流的持续时间紧密匹配。
