Borrell N, Acinas S G, Figueras M J, Martínez-Murcia A J
Departamento de Ciencias Médicas Básicas, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira y Virgili, Reus, Tarragona, Spain.
J Clin Microbiol. 1997 Jul;35(7):1671-4. doi: 10.1128/jcm.35.7.1671-1674.1997.
Identification of Aeromonas species, emergent pathogens for humans, has long been controversial due to their phenotypic and genomic heterogeneities. Computer analysis of the published 16S rRNA gene sequences revealed that restriction fragment length polymorphism of the PCR-amplified 16S rRNA gene is a good and rapid way of assessing the identities of all known species of Aeromonas. The method was evaluated with the reference strains of all species (or DNA homology groups) and 76 clinical isolates of diverse origin. Most results from the two approaches were in agreement, but some discrepancies were discerned. Advantages over previous phenotypic and genetic methods are discussed.
气单胞菌属是人类的新兴病原体,由于其表型和基因组的异质性,其鉴定一直存在争议。对已发表的16S rRNA基因序列进行计算机分析表明,PCR扩增的16S rRNA基因的限制性片段长度多态性是评估所有已知气单胞菌属物种身份的一种快速有效的方法。该方法用所有物种(或DNA同源组)的参考菌株和76株不同来源的临床分离株进行了评估。两种方法的大多数结果一致,但也发现了一些差异。文中讨论了该方法相对于以前的表型和基因方法的优势。