使用聚合酶链反应-探针试验鉴定舒氏气单胞菌和詹氏气单胞菌。

Identification of Aeromonas schubertii and Aeromonas jandaei by using a polymerase chain reaction-probe test.

作者信息

Ash C, Martinez-Murcia A J, Collins M D

机构信息

Department of Microbiology, Institute of Food Research, Reading, UK.

出版信息

FEMS Microbiol Lett. 1993 Apr 1;108(2):151-5. doi: 10.1111/j.1574-6968.1993.tb06091.x.

DOI:10.1111/j.1574-6968.1993.tb06091.x

PMID:8486241

Abstract

Two oligonucleotide primers were used in a polymerase chain reaction-protocol to amplify a region (approx. 850 bp) of the 16S rRNA gene of Aeromonas schubertii and Aeromonas jandaei. Hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method for the identification of these two species.

摘要

在聚合酶链反应方案中使用了两种寡核苷酸引物，以扩增舒氏气单胞菌和詹氏气单胞菌16S rRNA基因的一个区域（约850 bp）。聚合酶链反应产物与特异性内部探针的杂交提供了一种高度特异性的方法来鉴定这两个物种。