Rawe I M, Zabel R W, Tuft S J, Chen V, Meek K M
Open University, Oxford Research Unit, UK.
Eye (Lond). 1992;6 ( Pt 6):637-42. doi: 10.1038/eye.1992.137.
We have examined the morphology of the collagen and proteoglycans in rabbit corneas that have undergone excimer laser photorefractive keratectomy using a clinical, 193 nm excimer laser. The photoablation was carried out to a stromal depth of 100 microns and a diameter of 6 mm. All ablated corneas developed a haze that was most intense between week 4 and week 8 and which showed no improvement after week 16. The corneas were stained with the cationic dye cuprolinic blue to visualise proteoglycans and were then processed for transmission electron microscopy. The ultrastructural location of proteoglycans (keratan sulphate and dermatan sulphate) was observed in the corneal wounds at different time intervals. Corneas that had undergone steroid treatment post-operatively were also examined. In the healing tissue proteoglycan filaments of abnormal size were observed, which became most prominent after 2 weeks. As healing progressed these abnormal filaments decreased but after 45 weeks some were still present, indicating that the proteoglycan content had not returned to normal.
我们使用临床用的193纳米准分子激光，检查了接受准分子激光屈光性角膜切削术的兔角膜中胶原蛋白和蛋白聚糖的形态。光消融至基质深度100微米，直径6毫米。所有接受消融的角膜均出现了混浊，在第4周和第8周之间最为严重，在第16周后没有改善。角膜用阳离子染料铜叶绿酸蓝染色以观察蛋白聚糖，然后进行透射电子显微镜处理。在不同时间间隔观察角膜伤口中蛋白聚糖（硫酸角质素和硫酸皮肤素）的超微结构位置。还检查了术后接受类固醇治疗的角膜。在愈合组织中观察到大小异常的蛋白聚糖细丝，在2周后最为明显。随着愈合进展，这些异常细丝减少，但在45周后仍有一些存在，表明蛋白聚糖含量尚未恢复正常。
