Nakamura M, Sato N, Chikama T, Hasegawa Y, Nishida T
Department of Ophthalmology, Yamaguchi University School of Medicine, Ube City, Japan.
Exp Eye Res. 1997 Mar;64(3):355-9. doi: 10.1006/exer.1996.0216.
To determine whether the effect of fibronectin on corneal epithelial healing occurs in eyes affected by diabetes, the effect of fibronectin on the rates at which corneal epithelial wounds healed was compared in diabetic and normal rats. Streptozotocin was used to induce diabetes in rats. Two weeks after treatment, the whole corneal epithelium of diabetic and untreated rats was debrided. Fibronectin eye drops, at concentrations ranging from 0-01 to 1 mg ml-1, were given six times a day for three days, starting immediately after debridement. The area of the corneal epithelial wound was measured every 12 hr. Although the healing process was similar in normal and diabetic rats, the healing rate in diabetic rats was slower than that in normal controls after the 12 hr immediately following debridement. In both diabetic and normal rats, fibronectin eye drops reduced the wound area in a dose-dependent manner. The fibronectin dose of 1 mg ml-1 reduced the wound area significantly, compared with control eyes treated with phosphate-buffered saline. These results demonstrated that fibronectin facilitates corneal epithelial wound healing in diabetic rats.
为了确定纤连蛋白对角膜上皮愈合的影响是否发生在糖尿病患眼中，研究人员比较了纤连蛋白对糖尿病大鼠和正常大鼠角膜上皮伤口愈合速率的影响。用链脲佐菌素诱导大鼠患糖尿病。治疗两周后，去除糖尿病大鼠和未治疗大鼠的整个角膜上皮。清创后立即开始，每天六次给予浓度范围为0.01至1mg/ml的纤连蛋白滴眼液，持续三天。每12小时测量角膜上皮伤口的面积。虽然正常大鼠和糖尿病大鼠的愈合过程相似，但在清创后的12小时内，糖尿病大鼠的愈合速率比正常对照组慢。在糖尿病大鼠和正常大鼠中，纤连蛋白滴眼液均以剂量依赖的方式减少伤口面积。与用磷酸盐缓冲盐水处理的对照眼相比，1mg/ml的纤连蛋白剂量显著减少了伤口面积。这些结果表明，纤连蛋白促进糖尿病大鼠角膜上皮伤口的愈合。
