de Broin E, Urata K, Giroux L, Lepage R, Huet P M
Centre de recherche clinique André-Viallet, Département des Laboratoires, Hôpital Saint-Luc and Université de Montréal, Québec,Canada.
Transplantation. 1997 Jun 15;63(11):1547-54. doi: 10.1097/00007890-199706150-00002.
Nisoldipine, a calcium antagonist, has been reported to improve the quality of grafted rat livers. We thus assessed the protective effect of two calcium antagonists, nisoldipine and nickel, during extended cold ischemia-reperfusion.
Rat livers were isolated and perfused before or after 24 hr of cold ischemia in University of Wisconsin solution (4 degrees C) with or without nisoldipine or nickel. Sinusoidal endothelial cell and hepatocyte functions were measured by hyaluronic acid and taurocholate elimination, respectively.
Similar alterations in hepatocyte and sinusoidal cell functions were found in all groups after cold ischemia with or without calcium antagonists. In a second set of experiments, liver transplantation was performed in two groups of rats with livers stored under identical conditions with or without nisoldipine. Seven of 12 animals (62.5%) in both groups survived for over 10 days after 24-hr preservation in University of Wisconsin solution. Survival rates were similar in both groups.
Calcium antagonists do not appear to have a direct protective effect on sinusoidal endothelial cell and hepatocyte functions, nor on the overall liver preservation after extended cold preservation-reperfusion.
硝苯地平作为一种钙拮抗剂，据报道可改善移植大鼠肝脏的质量。因此，我们评估了两种钙拮抗剂硝苯地平和镍在延长冷缺血再灌注期间的保护作用。
将大鼠肝脏分离，在威斯康星大学溶液（4℃）中进行24小时冷缺血，然后在有或无硝苯地平或镍的情况下进行灌注。分别通过透明质酸和牛磺胆酸盐清除率来测定肝血窦内皮细胞和肝细胞的功能。
在有或无钙拮抗剂的冷缺血后，所有组的肝细胞和肝血窦细胞功能均出现类似改变。在另一组实验中，对两组大鼠进行肝脏移植，其肝脏在相同条件下保存，一组有硝苯地平，一组没有。在威斯康星大学溶液中保存24小时后，两组12只动物中有7只（62.5%）存活超过10天。两组的存活率相似。
钙拮抗剂似乎对肝血窦内皮细胞和肝细胞功能没有直接保护作用，对延长冷保存再灌注后的整体肝脏保存也没有直接保护作用。
