Tandon V, Pal P, Roy B, Rao H S, Reddy K S
Department of Zoology, North-Eastern Hill University, Shillong, India.
Parasitol Res. 1997;83(5):492-8. doi: 10.1007/s004360050286.
The in vitro activity of root-tuber-peel extract of Flemingia vestita, an indigenous plant consumed by the natives in Northeast India, was tested against helminth parasites. Live parasites (nematode: Ascaris suum from pigs, A. lumbricoides from humans, Ascaridia galli and Heterakis gallinarum from domestic fowl; cestode: Raillietina echinobothrida from domestic fowl; trematode: Paramphistomum sp. from cattle) were collected in 0.9 % physiological buffered saline (PBS) and maintained at 37 +/- 1 degrees C. In vitro treatment of the parasites with the crude extract (50 mg/ml) in PBS revealed complete immobilization of the trematode and cestode in about 43 and 20 min, respectively. However, the cuticle-covered nematodes did not show any change in physical activity and remained viable even after a long period of exposure to the extract. Exposure of R. echinobothrida to genistein (0.5 mg/ml), an active principle isolated from the root-tuber peel, caused spontaneous loss of movement (paralysis) in 4.5 h, which was slower than the time required for praziquantel, the reference flukicide and cestodicide. The treated parasites showed structural alteration in their tegumental architecture. This study suggests the vermifugal activity of this plant extract against trematodes and cestodes.
对印度东北部当地人食用的一种本土植物——长柄狸尾豆块根皮提取物的体外活性进行了抗蠕虫寄生虫测试。将活寄生虫（线虫：猪的猪蛔虫、人的蛔虫、家禽的鸡蛔虫和火鸡异刺线虫；绦虫：家禽的棘盘瑞利绦虫；吸虫：牛的同盘吸虫属）收集于0.9%生理缓冲盐水（PBS）中，并保持在37±1℃。在PBS中用粗提取物（50mg/ml）对寄生虫进行体外处理，结果显示吸虫和绦虫分别在约43分钟和20分钟内完全停止活动。然而，体表有角质层覆盖的线虫身体活动没有任何变化，即使长时间暴露于提取物中仍保持存活。将棘盘瑞利绦虫暴露于从块根皮中分离出的活性成分染料木黄酮（0.5mg/ml）中，4.5小时后出现自发运动丧失（麻痹），这比对照杀吸虫剂和杀绦虫剂吡喹酮所需的时间要长。经处理的寄生虫其体表结构出现改变。本研究表明该植物提取物对吸虫和绦虫具有驱虫活性。
