Fida R, Lyster D J, Bywater R A, Taylor G S
Department of Physiology, Monash University, Clayton, Victoria, Australia.
Neurogastroenterol Motil. 1997 Jun;9(2):99-107. doi: 10.1046/j.1365-2982.1997.d01-25.x.
Spontaneous contractions were recorded from the circular muscle layer at three sites along the isolated mouse colon. The interval between contractions was approximately 4.5 min. The mean duration of the contractions ranged from 26 sec in the distal colon to 45 sec in the proximal colon. Contractions migrating more than half the length of the colon were termed colonic migrating motor complexes (CMMCs). Over 90% of tissues demonstrated migration predominantly in an aboral direction. Hyoscine (10(-6) M) decreased the amplitude of the CMMCs by at least 40% but had no significant effect on the interval or duration of the CMMCs. Nifedipine (10(-6) M) significantly decreased the amplitude of the CMMCs by 95% but did not alter the duration or the interval between the CMMCs. Hexamethonium (5 x 10(-4) M) and tetrodotoxin (TTX; 2 x 10(-6) M) abolished all CMMC activity. TTX increased the resting tone of the preparations. Nitro-L-arginine (10(-4) M) increased the resting tone of the preparations and significantly decreased the interval between the CMMCs by approximately 80% but had no significant effect on the duration of the CMMCs. The results suggest CMMCs migrate predominantly in an aboral direction and are neurogenic in origin. Nitric oxide may be involved in maintaining inhibition of the muscle between CMMCs.
在分离的小鼠结肠的三个部位记录了环形肌层的自发收缩。收缩间隔约为4.5分钟。收缩的平均持续时间从远端结肠的26秒到近端结肠的45秒不等。迁移超过结肠长度一半的收缩被称为结肠移行性运动复合波（CMMCs）。超过90%的组织显示迁移主要沿口-肛方向。东莨菪碱（10^(-6) M）使CMMCs的幅度至少降低40%，但对CMMCs的间隔或持续时间没有显著影响。硝苯地平（10^(-6) M）使CMMCs的幅度显著降低95%，但未改变CMMCs的持续时间或间隔。六甲铵（5×10^(-4) M）和河豚毒素（TTX；2×10^(-6) M）消除了所有CMMC活动。TTX增加了标本的静息张力。硝基-L-精氨酸（10^(-4) M）增加了标本的静息张力，并使CMMCs之间的间隔显著缩短约80%，但对CMMCs的持续时间没有显著影响。结果表明，CMMCs主要沿口-肛方向迁移，起源于神经源性。一氧化氮可能参与维持CMMCs之间肌肉的抑制作用。
