Giesen K, Hummel T, Stollewerk A, Harrison S, Travers A, Klämbt C
Institut für Entwicklungsbiologie, Universität zu Köln, Germany.
Development. 1997 Jun;124(12):2307-16. doi: 10.1242/dev.124.12.2307.
Two classes of glial cells are found in the embryonic Drosophila CNS, midline glial cells and lateral glial cells. Midline glial development is triggered by EGF-receptor signalling, whereas lateral glial development is controlled by the gcm gene. Subsequent glial cell differentiation depends partly on the pointed gene. Here we describe a novel component required for all CNS glia development. The tramtrack gene encodes two zinc-finger proteins, one of which, ttkp69, is expressed in all non-neuronal CNS cells. We show that ttkp69 is downstream of gcm and can repress neuronal differentiation. Double mutant analysis and coexpression experiments indicate that glial cell differentiation may depend on a dual process, requiring the activation of glial differentiation by pointed and the concomitant repression of neuronal development by tramtrack.
在胚胎期果蝇的中枢神经系统中发现了两类神经胶质细胞，即中线神经胶质细胞和外侧神经胶质细胞。中线神经胶质细胞的发育由表皮生长因子受体信号触发，而外侧神经胶质细胞的发育则由gcm基因控制。随后的神经胶质细胞分化部分取决于pointed基因。在此，我们描述了中枢神经系统所有神经胶质细胞发育所需的一种新成分。tramtrack基因编码两种锌指蛋白，其中一种，ttkp69，在所有非神经元的中枢神经系统细胞中表达。我们表明，ttkp69在gcm下游，并且可以抑制神经元分化。双突变分析和共表达实验表明，神经胶质细胞的分化可能依赖于一个双重过程，需要通过pointed激活神经胶质细胞分化，同时通过tramtrack抑制神经元发育。
