一株新的沙眼衣原体分离株的特性，该分离株缺乏常见质粒并具有沙眼生物变种的特性。
Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma.
作者信息
Farencena A, Comanducci M, Donati M, Ratti G, Cevenini R
机构信息
Sezione di Microbiologia, DMCSS, Policlinico S. Orsola, Università di Bologna, Italy.
出版信息
Infect Immun. 1997 Jul;65(7):2965-9. doi: 10.1128/iai.65.7.2965-2969.1997.
Abstract
A Chlamydia trachomatis urethral isolate, alpha/95, yielding pgp3-negative but otherwise normal inclusions by immunofluorescence also gave negative results when pCT-homologous DNA was searched by PCR and Southern blotting. omp-1 sequence analysis identified alpha/95 as a new genotype B variant. These findings confirm that pCT is not required for chlamydial growth in vitro.
摘要
一株沙眼衣原体尿道分离株alpha/95，通过免疫荧光检测产生的包涵体pgp3呈阴性，但其他方面正常，当通过聚合酶链反应（PCR）和Southern印迹法检测pCT同源DNA时，也得到阴性结果。omp-1序列分析确定alpha/95为新的B基因型变体。这些发现证实，衣原体体外生长不需要pCT。
相似文献
1
Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma.
Infect Immun. 1997 Jul;65(7):2965-9. doi: 10.1128/iai.65.7.2965-2969.1997.
2
Characterization and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis.
Plasmid. 1987 Nov;18(3):205-14. doi: 10.1016/0147-619x(87)90063-1.
3
[Detection of Chlamydia trachomatis by PCR and genotyping in Moroccan trachoma patients].
Rev Int Trach Pathol Ocul Trop Subtrop Sante Publique. 1995;72:121-35.
4
Detection of infections of the eye with Chlamydia trachomatis by the polymerase chain reaction.
Int Ophthalmol. 1993;17(6):327-30. doi: 10.1007/BF00915738.
5
Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants.
J Infect Dis. 1992 Nov;166(5):1173-7. doi: 10.1093/infdis/166.5.1173.
6
Diagnosis of Chlamydia trachomatis eye infection in Tanzania by polymerase chain reaction/enzyme immunoassay.
Lancet. 1991 Oct 5;338(8771):847-50. doi: 10.1016/0140-6736(91)91502-l.
7
Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to Invasive Biovars.
mBio. 2018 Jan 30;9(1):e02164-17. doi: 10.1128/mBio.02164-17.
8
Genetic transformation of a clinical (genital tract), plasmid-free isolate of Chlamydia trachomatis: engineering the plasmid as a cloning vector.
PLoS One. 2013;8(3):e59195. doi: 10.1371/journal.pone.0059195. Epub 2013 Mar 18.
9
Plaque formation by and plaque cloning of Chlamydia trachomatis biovar trachoma.
J Clin Microbiol. 1998 Oct;36(10):3013-9. doi: 10.1128/JCM.36.10.3013-3019.1998.
10
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain.
BMC Genomics. 2009 May 21;10:239. doi: 10.1186/1471-2164-10-239.
引用本文的文献
1
Analytical and clinical validation of a multiplex PCR assay for detection of and including simultaneous LGV serotyping on an automated high-throughput PCR system.
Microbiol Spectr. 2024 Mar 5;12(3):e0275623. doi: 10.1128/spectrum.02756-23. Epub 2024 Feb 12.
2
Plasmid-mediated virulence in .
Front Cell Infect Microbiol. 2023 Aug 17;13:1251135. doi: 10.3389/fcimb.2023.1251135. eCollection 2023.
3
Infection Remodels Host Cell Mitochondria to Alter Energy Metabolism and Subvert Apoptosis.
Microorganisms. 2023 May 24;11(6):1382. doi: 10.3390/microorganisms11061382.
4
Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells.
BMC Microbiol. 2023 Mar 4;23(1):58. doi: 10.1186/s12866-023-02802-3.
5
Chlamydia overcomes multiple gastrointestinal barriers to achieve long-lasting colonization.
Trends Microbiol. 2021 Nov;29(11):1004-1012. doi: 10.1016/j.tim.2021.03.011. Epub 2021 Apr 14.
6
The Nature and Extent of Plasmid Variation in .
Microorganisms. 2020 Mar 6;8(3):373. doi: 10.3390/microorganisms8030373.
7
Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes.
mBio. 2019 Mar 19;10(2):e00225-19. doi: 10.1128/mBio.00225-19.
8
High prevalence of Chlamydia trachomatis in pregnant women attended at Primary Health Care services in Amazon, Brazil.
Rev Inst Med Trop Sao Paulo. 2019 Feb 14;61:e6. doi: 10.1590/S1678-9946201961006.
9
Genetic Transformation of a Ocular Isolate With the Functional Tryptophan Synthase Operon Confers an Indole-Rescuable Phenotype.
Front Cell Infect Microbiol. 2018 Dec 14;8:434. doi: 10.3389/fcimb.2018.00434. eCollection 2018.
10
Genomic analyses of the Chlamydia trachomatis core genome show an association between chromosomal genome, plasmid type and disease.
BMC Genomics. 2018 Feb 9;19(1):130. doi: 10.1186/s12864-018-4522-3.
本文引用的文献
1
Differences in the envelope proteins of Chlamydia pneumoniae, Chlamydia trachomatis, and Chlamydia psittaci shown by two-dimensional gel electrophoresis.
Arch Microbiol. 1996 Mar;165(3):164-8. doi: 10.1007/BF01692857.
2
Expression of a plasmid gene of Chlamydia trachomatis encoding a novel 28 kDa antigen.
J Gen Microbiol. 1993 May;139(5):1083-92. doi: 10.1099/00221287-139-5-1083.
3
Expression of recombinant DNA introduced into Chlamydia trachomatis by electroporation.
Can J Microbiol. 1994 Jul;40(7):583-91. doi: 10.1139/m94-093.
4
Characterization of the humoral response induced by a synthetic peptide of the major outer membrane protein of Chlamydia trachomatis serovar B.
Infect Immun. 1994 Aug;62(8):3547-9. doi: 10.1128/iai.62.8.3547-3549.1994.
5
A comprehensive set of sequence analysis programs for the VAX.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
6
Diversity of Chlamydia trachomatis major outer membrane protein genes.
J Bacteriol. 1987 Sep;169(9):3879-85. doi: 10.1128/jb.169.9.3879-3885.1987.
7
Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars.
Infect Immun. 1989 Apr;57(4):1040-9. doi: 10.1128/iai.57.4.1040-1049.1989.
8
Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes.
Proc Natl Acad Sci U S A. 1988 Jun;85(11):4000-4. doi: 10.1073/pnas.85.11.4000.
9
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
10
The 7.5-kb plasmid present in Chlamydia trachomatis is not essential for the growth of this microorganism.
Plasmid. 1990 Mar;23(2):144-8. doi: 10.1016/0147-619x(90)90033-9.
Zotero 插件