Schepens J, Cuppen E, Wieringa B, Hendriks W
Department of Cell Biology and Histology, Institute of Cellular Signalling, University of Nijmegen, The Netherlands.
FEBS Lett. 1997 Jun 2;409(1):53-6. doi: 10.1016/s0014-5793(97)00481-x.
PDZ motifs are small protein-protein interaction modules that are thought to play a role in the clustering of submembranous signalling molecules. The specificity and functional consequences of their associative actions is still largely unknown. Using two-hybrid methodology we here demonstrate that the PDZ motif of neuronal nitric oxide synthase (nNOS) can mediate the binding to several other proteins in brain. Peptide library screening showed that proteins bearing a carboxy-terminal G(D,E)XV* sequence are preferred targets for the nNOS amino-terminal PDZ motif. Potential nNOS targets include a melanoma-associated antigen, cyclophilins and the alpha1C-adrenergic receptor.
PDZ基序是小的蛋白质-蛋白质相互作用模块，被认为在膜下信号分子的聚集过程中发挥作用。其缔合作用的特异性和功能后果在很大程度上仍不清楚。我们在此利用双杂交方法证明，神经元型一氧化氮合酶（nNOS）的PDZ基序可介导与脑中其他几种蛋白质的结合。肽库筛选表明，带有羧基末端G(D,E)XV*序列的蛋白质是nNOS氨基末端PDZ基序的优先靶标。nNOS的潜在靶标包括一种黑色素瘤相关抗原、亲环蛋白和α1C-肾上腺素能受体。
