Nakao K, Imoto I, Gabazza E C, Yamauchi K, Yamazaki N, Taguchi Y, Shibata T, Takaji S, Ikemura N, Misaki M
Third Dept. of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
Scand J Gastroenterol. 1997 Jun;32(6):530-4. doi: 10.3109/00365529709025094.
Recently, in vitro studies suggested that lactoferrin (Lf) might play an important role in the physiopathology of Helicobacter pylori-associated gastritis. However, whether Lf is present in the gastric juice and its relationship with H. pylori infection have not as yet been reported. In the present investigation the presence of Lf in gastric juice and its correlation with H. pylori infection were assessed.
This study comprised 30 H. pylori-positive and 14-negative patients with chronic gastritis. Gastric juice levels of Lf were measured with enzyme-linked immunoassays. Gastric juice concentration of Lf was also investigated in accordance with the histologic findings of biopsy specimens in the gastric body and antrum.
Lf concentration in gastric juice was significantly higher in H. pylori-positive than in -negative patients (P = 0.033). The pH values are known to influence the levels of Lf. However, intragastric Lf levels were also significantly increased in H. pylori-positive patients as compared with H. pylori-negative patients after correcting the Lf levels for pH values (P = 0.029) or after adjusting the pH values of the gastric juice with NaHCO3 solution in both groups of patients (P = 0.0007). In addition, the gastric juice levels of Lf correlated significantly with the gastric mucosal concentrations of Lf in the gastric body (P < 0.005, r = 0.568) and the antrum (P < 0.05, r = 0.401).
This study showed for the first time that Lf is present in gastric juice and that it correlates with H. pylori infection. Lf may constitute a good marker for H. pylori-associated gastritis. Although correlation does not prove causation, this study suggests that Lf might play an important role in the physiopathology of H. pylori-associated gastritis.
最近，体外研究表明乳铁蛋白（Lf）可能在幽门螺杆菌相关性胃炎的病理生理过程中发挥重要作用。然而，胃液中是否存在Lf及其与幽门螺杆菌感染的关系尚未见报道。在本研究中，对胃液中Lf的存在情况及其与幽门螺杆菌感染的相关性进行了评估。
本研究纳入了30例幽门螺杆菌阳性和14例阴性的慢性胃炎患者。采用酶联免疫吸附测定法测量胃液中Lf的水平。还根据胃体和胃窦活检标本的组织学结果研究了胃液中Lf的浓度。
幽门螺杆菌阳性患者胃液中Lf浓度显著高于阴性患者（P = 0.033）。已知pH值会影响Lf水平。然而，在对pH值校正Lf水平后（P = 0.029），或在两组患者中用碳酸氢钠溶液调节胃液pH值后（P = 0.0007），幽门螺杆菌阳性患者胃内Lf水平仍显著高于幽门螺杆菌阴性患者。此外，胃液中Lf水平与胃体（P < 0.005，r = 0.568）和胃窦（P < 0.05，r = 0.401）胃黏膜中Lf浓度显著相关。
本研究首次表明胃液中存在Lf，且其与幽门螺杆菌感染相关。Lf可能是幽门螺杆菌相关性胃炎的良好标志物。虽然相关性并不证明因果关系，但本研究表明Lf可能在幽门螺杆菌相关性胃炎的病理生理过程中发挥重要作用。
