Voss H, Benes V, Andrade M A, Valencia A, Rechmann S, Teodoru C, Schwager C, Paces V, Sander C, Ansorge W
Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Yeast. 1997 Jun 15;13(7):655-72. doi: 10.1002/(SICI)1097-0061(19970615)13:7<655::AID-YEA120>3.0.CO;2-I.
We have determined the nucleotide sequence of 129,524 bases of yeast (Saccharomyces cerevisiae) chromosome XV. Sequence analysis revealed the presence of 59 non-overlapping open reading frames (ORFs) of length > 300 bp, three tRNA genes, four delta elements and one Ty-element. Among the 21 previously known yeast genes (36% of all ORFs in this fragment) were nucleoporin (NUP1), ras protein (RAS1), RNA polymerase III (RPC1) and elongation factor 2 (EF2). Further, 31 ORFs (53% of the total) were found to be homologous to known protein or DNA sequences, or sequence patterns. For seven ORFs (11% of the total) no homology was found. Among the most interesting protein identification in this DNA fragment are an inositol polyphosphatase, the second gene of this type found in yeast (homologous to the human OCRL gene involved in Lowe's syndrome), a new ADP ribosylation factor of the arf6 subfamily, the first protein containing three C2 domains, and an ORF similar to a Bacillus subtilis cell-cycle related protein. For each ORF detailed sequence analysis was carried out, with a full consideration of its biological function and pointing out key regions of interest for further functional analysis.
我们已经确定了酵母（酿酒酵母）第十五号染色体129,524个碱基的核苷酸序列。序列分析显示存在59个长度大于300 bp的非重叠开放阅读框（ORF）、三个tRNA基因、四个δ元件和一个Ty元件。在21个先前已知的酵母基因（该片段中所有ORF的36%）中，有核孔蛋白（NUP1）、ras蛋白（RAS1）、RNA聚合酶III（RPC1）和延伸因子2（EF2）。此外，发现31个ORF（占总数的53%）与已知的蛋白质或DNA序列或序列模式同源。有七个ORF（占总数的11%）未发现同源性。在该DNA片段中最有趣的蛋白质鉴定结果包括一种肌醇多磷酸酶，这是在酵母中发现的该类型的第二个基因（与涉及劳氏综合征的人类OCRL基因同源）、arf6亚家族的一种新的ADP核糖基化因子、第一个含有三个C2结构域的蛋白质，以及一个与枯草芽孢杆菌细胞周期相关蛋白相似的ORF。对每个ORF都进行了详细的序列分析，充分考虑了其生物学功能，并指出了进一步功能分析感兴趣的关键区域。
