Ziprin R L
USDA, Agricultural Research Service, College Station, Texas 77845, USA.
Avian Dis. 1997 Apr-Jun;41(2):438-41.
The present work compared the accumulation of intraperitoneal heterophils in day-of-hatch chicks following treatment with Salmonella enteritidis-immune lymphokine (ILK) and challenge with various strains of Salmonella enteritidis (SE). Day-of-hatch chicks received ILK by intraperitoneal injection and were challenged 1 hr later by intraperitoneal inoculation with one of the following SE strains: a wild-type, SE 890034-3; a delta cya-12 delta cyp-11 avirulent vaccine strain, chi 4357; and an invasion-deficient strain, InvA::kan, chi 4420. Four hours after challenge heterophils were recovered from the peritoneal cavity by lavage. The concentration of heterophils in the recovered lavage fluid was determined. Heterophil concentrations increased in response to challenge with each SE strain but there was a lower response to the invasion-deficient strain. The difference was statistically significant. This diminished heterophil response to challenge with invasion-deficient salmonellae supports existing evidence that the initial defensive reaction occurs at the earliest stages of the Salmonella-host interaction.
本研究比较了用肠炎沙门氏菌免疫淋巴细胞因子（ILK）处理后并经不同肠炎沙门氏菌（SE）菌株攻毒的出壳雏鸡腹腔内嗜异性粒细胞的蓄积情况。出壳雏鸡通过腹腔注射接受ILK，并在1小时后通过腹腔接种以下SE菌株之一进行攻毒：野生型SE 890034-3；缺失cya-12和cyp-11的无毒疫苗菌株chi 4357；以及侵袭缺陷菌株InvA::kan，chi 4420。攻毒4小时后，通过灌洗从腹腔中回收嗜异性粒细胞。测定回收的灌洗液中嗜异性粒细胞的浓度。每种SE菌株攻毒后嗜异性粒细胞浓度均升高，但对侵袭缺陷菌株的反应较低。差异具有统计学意义。对侵袭缺陷型沙门氏菌攻毒的嗜异性粒细胞反应减弱，支持了现有证据，即最初的防御反应发生在沙门氏菌与宿主相互作用的最早阶段。
