McGruder E D, Ramirez G A, Kogut M H, Moore R W, Corrier D E, Deloach J R, Hargis B M
Department of Poultry Science, Texas A&M University, College Station 77843.
Poult Sci. 1995 Jan;74(1):18-25. doi: 10.3382/ps.0740018.
We previously reported that the prophylactic, intraperitoneal administration of supernatants from concanavalin A-stimulated T cells derived from Salmonella enteritidis (SE)-immune White Leghorn hens (i.e., SE-immune lymphokines or ILK), conferred protection to neonatal White Leghorn chicks against SE organ invasion. In the present study, we evaluated the effects of in ovo administration of ILK on hatchability, hatch weight, in vitro bactericidal activity of heterophils, and protection against SE organ invasion in neonatal White Leghorn chicks. On Day 18 of embryogenesis, injections were made into the amnion with either ILK or nonimmune ILK (NILK) or were not injected (untreated). On the day of hatch, whole blood was collected from 20 of the chicks per treatment group for heterophil isolation. All remaining chicks were orally challenged with 5 x 10(4) cfu SE. Twenty-four hours after SE challenge, organs (liver and spleen) from the chicks were cultured for SE. Hatchability of ILK- and NILK-treated chicks was not different from that of untreated chicks. Hatch weights of ILK-treated chicks were approximately 1 g less (P < .05) than that of NILK-treated or untreated chicks. In vitro bactericidal activity of peripheral blood heterophils derived from ILK-treated chicks was increased (P < .05) above activity from heterophils derived from NILK-treated or untreated chicks. Organ invasion with SE was markedly and significantly decreased in the ILK-treated chicks as compared with chicks treated with NILK or untreated chicks. These results suggest that in ovo administration of ILK confers protection to neonatal chicks against SE organ infectivity at hatch.
我们之前报道过，腹腔注射来自经肠炎沙门氏菌（SE）免疫的白来航鸡的伴刀豆球蛋白A刺激的T细胞的上清液（即SE免疫淋巴细胞因子或ILK），可保护新生白来航鸡免受SE的器官侵袭。在本研究中，我们评估了在鸡胚内注射ILK对孵化率、出壳重、嗜异性粒细胞的体外杀菌活性以及新生白来航鸡抵抗SE器官侵袭的影响。在胚胎发育第18天，向羊膜腔内注射ILK或非免疫ILK（NILK），或不进行注射（未处理）。在出壳当天，从每个处理组的20只雏鸡采集全血以分离嗜异性粒细胞。所有其余雏鸡口服接种5×10⁴cfu的SE。SE攻毒24小时后，对雏鸡的器官（肝脏和脾脏）进行SE培养。ILK处理组和NILK处理组雏鸡的孵化率与未处理组雏鸡的孵化率没有差异。ILK处理组雏鸡的出壳重比NILK处理组或未处理组雏鸡的出壳重轻约1g（P<0.05）。与NILK处理组或未处理组雏鸡来源的嗜异性粒细胞相比，ILK处理组雏鸡来源的外周血嗜异性粒细胞的体外杀菌活性增强（P<0.05）。与NILK处理组雏鸡或未处理组雏鸡相比，ILK处理组雏鸡中SE的器官侵袭明显且显著减少。这些结果表明，在鸡胚内注射ILK可使新生雏鸡在出壳时免受SE的器官感染。
