Levis C, Fortini D, Brygoo Y
Station de Pathologie Végétale, INRA, Route de Saint Cyr, Versailles,France.
Mol Gen Genet. 1997 May;254(6):674-80. doi: 10.1007/s004380050465.
A transposable element, Flipper, was isolated from the phytopathogenic fungus Botrytis cinerea. The element was identified as an insertion sequence within the coding region of the nitrate reductase gene. The Flipper sequence is 1842 bp long with perfect inverted terminal repeats (ITRs) of 48 bp and an open reading frame (ORF) of 533 amino acids, potentially encoding for a transposase; the element is flanked by the dinucleotide TA. The encoded protein is very similar to the putative transposases of three elements from other phytopathogenic fungi, Fot1 from Fusarium oxysporum, and Pot2 and MGR586 from Magnaporthe grisea. The number of Flipper elements in strains of B. cinerea varied from 0 to 20 copies per genome. Analysis of the descendants of one cross showed that the segregation ratio of Flipper elements was 2:2 and that the copies were not linked.
从植物病原真菌灰葡萄孢中分离出一种转座元件Flipper。该元件被鉴定为硝酸还原酶基因编码区内的一个插入序列。Flipper序列长1842 bp，具有48 bp的完美反向末端重复序列（ITR）和一个533个氨基酸的开放阅读框（ORF），可能编码一种转座酶；该元件两侧为二核苷酸TA。编码的蛋白质与其他植物病原真菌的三种元件（尖孢镰刀菌的Fot1、稻瘟病菌的Pot2和MGR586）的推定转座酶非常相似。灰葡萄孢菌株中Flipper元件的数量在每个基因组0至20个拷贝之间变化。对一次杂交后代的分析表明，Flipper元件的分离比为2:2，且这些拷贝没有连锁。
