Rauch F, Middelmann B, Cagnoli M, Keller K M, Schönau E
Kinderklinik, Universität zu Köln, Germany.
Acta Paediatr. 1997 Jun;86(6):583-7. doi: 10.1111/j.1651-2227.1997.tb08938.x.
We compared serum levels of total alkaline phosphatase (TAP) and bone-specific alkaline phosphatase (BAP) as determined by three different assays (lectin affinity electrophoresis, immunoradiometric assay, enzyme-linked immunosorbent assay) in subjects aged 5-20 years suffering from X-linked hypophosphatemic rickets (n = 14), chronic renal failure (n = 10) and chronic cholestatic liver disease (n = 16). Results were compared to controls of the same age and were expressed as standard deviation scores (SDS). TAP correlated significantly with BAP (r > 0.9 for each assay; p < 0.001) in controls. In children with cholestatic diseases, TAP (median SDS + 2.0) was elevated, but BAP, as measured by the electrophoretic assay, was within the reference range for most patients (median SDS: -0.4; p = 0.003 for the difference between the median SDS of TAP and BAP). In contrast, results for BAP as determined by the two immunoassays were not significantly different from TAP in any of the three patient groups (p > 0.05 in each group for both assays). In this study, the two immunoassays did not have a detectable advantage over lectin affinity electrophoresis in the determination of BAP.
我们比较了年龄在5至20岁的患有X连锁低磷血症性佝偻病（n = 14）、慢性肾衰竭（n = 10）和慢性胆汁淤积性肝病（n = 16）的受试者中，通过三种不同检测方法（凝集素亲和电泳、免疫放射分析、酶联免疫吸附测定）测定的血清总碱性磷酸酶（TAP）和骨特异性碱性磷酸酶（BAP）水平。将结果与相同年龄的对照组进行比较，并表示为标准差评分（SDS）。在对照组中，TAP与BAP显著相关（每种检测方法的r>0.9；p<0.001）。在胆汁淤积性疾病患儿中，TAP升高（中位数SDS + 2.0），但通过电泳检测法测得的BAP，大多数患者在参考范围内（中位数SDS：-0.4；TAP和BAP的中位数SDS差异p = 0.003）。相比之下，在三个患者组中的任何一组中，通过两种免疫测定法测定的BAP结果与TAP均无显著差异（两种测定法在每组中的p>0.05）。在本研究中，在测定BAP方面，两种免疫测定法相对于凝集素亲和电泳没有可检测到的优势。
