Department of Food and Nutrition, Keimyung University, Daegu, South Korea.
Adv Exp Med Biol. 2017;975 Pt 2:1093-1102. doi: 10.1007/978-94-024-1079-2_87.
Taurine is thought to affect bone in rats favorably. However, studies on the actions of this estrogen deficiency and high cholesterol diet factors on the bone metabolism are limited. In this study, the protective effect of taurine on bone was determined. Thirty-two 42 days old female SD rats were placed in individual stainless cages. Given to rats was fed to chow (Samyang Corporation, South Korea) and deionized water for a 4 days adaptation period. After the period of adaptation, Half of the rats were induced estrogen deficiency model by ovariectomy (OVX), and the left rats with sham-operated were used control (SHAM). For six weeks, the OVX and SHAM rats had separately a 2% taurine supplemented diet with ad libitum in both the water and the food. DEXA for small animals (PIXImus, GE Lunar co, Wisconsin) was used to determine spinal and femoral bone. The concentrations of serum calcium and phosphorus were also measured. The monitoring of bone formation was done by determining the serum ALP and osteocalcin. Urinary DPD the values were determined as index of bone resorption. Statistical measure was done with SAS (version 9.3). A lower overall intake of the daily food was observed in non-ovariectomized rats than in the OVX rats. At sacrifice, a much greater body weight was observed in ovariectomized group compare to non-operated group. That difference was absent in both fed taurine SHAM and OVX rats. Serum calcium and phosphorus were not statistically different by taurine supplementation. Urinary excretion of calcium was not effected by taurine supplementation. Serum ALP and was significantly decreased by taurine in OVX rats (p < 0.05). For the spine BMD and BMC, there was no difference among SHAM and OVX rats by taurine. Spine BMC per body weight of taurine groups were higher than control groups (p < 0.1). No significant difference was observed after taurine supplementation in femur BMD and BMC. The analysis of the results suggest that taurine supplementation modulates the bone mineral contents in postmenopausal model rats fed with high cholesterol diet.
牛磺酸被认为对大鼠的骨骼有积极影响。然而，关于这种雌激素缺乏和高胆固醇饮食因素对骨骼代谢的作用的研究有限。在这项研究中，确定了牛磺酸对骨骼的保护作用。32 只 42 天大的 SD 雌性大鼠被放置在单独的不锈钢笼中。大鼠给予饲料（Samyang Corporation，韩国）和去离子水适应 4 天。适应期后，一半大鼠通过卵巢切除术（OVX）诱导雌激素缺乏模型，左侧假手术大鼠作为对照（SHAM）。六周内，OVX 和 SHAM 大鼠分别在水和食物中自由摄入 2%牛磺酸补充饮食。小动物 DEXA（PIXImus，GE Lunar co，威斯康星州）用于确定脊柱和股骨的骨密度。还测量了血清钙和磷的浓度。通过测定血清碱性磷酸酶（ALP）和骨钙素来监测骨形成。尿 DPD 值被确定为骨吸收的指标。统计测量使用 SAS（版本 9.3）进行。非卵巢切除大鼠的每日食物总摄入量低于卵巢切除大鼠。在处死时，卵巢切除组的体重明显大于非手术组。在给予牛磺酸的 SHAM 和 OVX 大鼠中，这种差异不存在。牛磺酸补充对血清钙和磷没有统计学上的差异。牛磺酸补充不影响尿钙排泄。血清 ALP 在 OVX 大鼠中显著降低（p < 0.05）。对于脊柱 BMD 和 BMC，SHAM 和 OVX 大鼠之间没有差异。牛磺酸组的脊柱 BMC 与体重的比值高于对照组（p < 0.1）。牛磺酸补充后，股骨 BMD 和 BMC 无显著差异。结果分析表明，牛磺酸补充剂可调节高胆固醇饮食喂养的绝经后模型大鼠的骨矿物质含量。
