Zeng H, MacAulay C, McLean D I, Palcic B
Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, Canada.
J Photochem Photobiol B. 1997 Apr;38(2-3):234-40. doi: 10.1016/s1011-1344(96)00008-5.
The in vivo skin autofluorescence spectrum was reconstructed by Monte Carlo simulation using microscopic fluorophore distributions and intrinsic fluorescence spectra measured from excised skin tissue sections as well as employing published skin tissue optical parameters. The theoretical modeling took into account the light-tissue interactions of scattering, absorption, and regeneration of fluorescence photons. The modification of the intrinsic spectra by tissue optical properties to generate the in vivo spectrum observed at the tissue surface can be represented by a fluorescence detection efficiency function (eta) which equals the integral of the product of the excitation light distribution inside the tissue and the fluorescence escape efficiency. Comparison of the reconstructed in vivo spectrum with the measured spectra showed good agreement, outside of the blood absorption bands, suggesting that (i) the theoretical modeling, (ii) the skin optical parameters used, and (iii) the measured microscopic morphology and spectral data are consistent. The divergence which exists over the strong blood absorption wavelength band (530-600 nm) suggests that the effect of blood contents on in vivo tissue optical properties deserves further investigations.
通过蒙特卡罗模拟重建体内皮肤自体荧光光谱，该模拟使用从切除的皮肤组织切片测量的微观荧光团分布和固有荧光光谱，并采用已发表的皮肤组织光学参数。理论建模考虑了荧光光子的散射、吸收和再生等光与组织的相互作用。组织光学特性对固有光谱的修正，以生成在组织表面观察到的体内光谱，可用荧光检测效率函数（η）表示，该函数等于组织内激发光分布与荧光逃逸效率乘积的积分。重建的体内光谱与测量光谱的比较表明，在血液吸收带之外两者吻合良好，这表明（i）理论建模，（ii）所使用的皮肤光学参数，以及（iii）测量的微观形态和光谱数据是一致的。在强血液吸收波长带（530 - 600 nm）上存在的差异表明，血液含量对体内组织光学特性的影响值得进一步研究。
