Grönlund-Pakkanen S, Mäkinen K, Talja M, Kuusisto A, Alhava E
Department of Surgery, Kuopio University Hospital, Finland.
J Photochem Photobiol B. 1997 Apr;38(2-3):269-73. doi: 10.1016/s1011-1344(96)00007-3.
Photodynamic therapy (PDT) is a new anticancer technique directed at the selective destruction of neoplastic tissue. Aminolevulinic acid (ALA), a precursor in the biosynthesis of home, induces the production of the endogenous photosensitizer protoporphyrin IX (PpIX). In this study the fluorescence distribution of ALA-induced PpIX was investigated in the rat bladder wall by fluorescence microscopy. The fluorescence studies showed that PpIX concentrates in bladder mucosa and that the highest fluorescence levels are achieved after four hours of 300 mg/kg ALA administration. A clear trend in difference between mucosa and muscularis layers was found in all samples taken after 1, 2, 4 and 6 hours of ALA administration. Our results suggest that to get the highest PpIX fluorescence intensity in bladder mucosa it is best to use 300 mg/kg ALA administration. Four hours is the time point when the highest difference in fluorescence between mucosa and muscularis is reached.
光动力疗法（PDT）是一种针对肿瘤组织进行选择性破坏的新型抗癌技术。氨基乙酰丙酸（ALA）是血红素生物合成的前体，可诱导内源性光敏剂原卟啉IX（PpIX）的产生。在本研究中，通过荧光显微镜研究了ALA诱导的PpIX在大鼠膀胱壁中的荧光分布。荧光研究表明，PpIX集中在膀胱黏膜中，在给予300mg/kg ALA四小时后达到最高荧光水平。在给予ALA 1、2、4和6小时后采集的所有样本中，均发现黏膜层和肌层之间存在明显的差异趋势。我们的结果表明，为了在膀胱黏膜中获得最高的PpIX荧光强度，最好使用300mg/kg的ALA给药剂量。四小时是黏膜和肌层之间荧光差异达到最大的时间点。
