Mäkinen K, Grönlund-Pakkanen S, Tiirikainen M, Nuutinen P, Kuusisto A, Alhava E
Dept. of Surgery, Kuopio University Hospital, Finland.
Scand J Gastroenterol. 1997 Jul;32(7):633-7. doi: 10.3109/00365529708996510.
Photodynamic therapy (PDT) is a method for local and selective tumour destruction achieved by the action of light on a photosensitizing drug.
We investigated the distribution of 5-amino-laevulinic acid (ALA)-induced protoporphyrin-IX fluorescence in rat oesophagus by fluorescence microscopic examination and then studied the effects of PDT.
The highest level of fluorescence was achieved in the mucosa after 4 h of 300 mg/kg ALA administration. A clear difference in fluorescence between mucosa and muscularis was found in all samples except those taken 24 h after ALA administration. PDT with ALA caused destruction of the mucosal and, partly, submucosal layers of the oesophagus without damaging the muscularis layer.
According to our results with microscopic fluorescence kinetics and the preliminary results of PDT, selective destruction of the superficial layer of the rat oesophagus is achieved with PDT after ALA administration.
光动力疗法（PDT）是一种通过光作用于光敏药物实现局部和选择性肿瘤破坏的方法。
我们通过荧光显微镜检查研究了5-氨基-γ-酮戊酸（ALA）诱导的原卟啉-IX荧光在大鼠食管中的分布，然后研究了光动力疗法的效果。
给予300mg/kg ALA 4小时后，黏膜中的荧光水平最高。除ALA给药24小时后采集的样本外，所有样本的黏膜和肌层之间荧光均有明显差异。ALA光动力疗法导致食管黏膜层和部分黏膜下层破坏，而肌层未受损。
根据我们的微观荧光动力学研究结果和光动力疗法的初步结果，ALA给药后光动力疗法可实现大鼠食管表层的选择性破坏。
