Li H, He B, Que C, Weng B
Department of Respiratory Medicine, First Affiliated Hospital of Beijing Medical University.
Chin Med J (Engl). 1996 Jul;109(7):533-6.
To investigate the influence of alveolar macrophages (AMs), fibroblasts and interstitial cells on development of lung fibrosis, and the interactions among TGF-beta 1 PDGF and IGF-1 and these cytokines-effects on lung fibrosis.
Expressions of TGF-beta 1, PDGF and IGF-1 mRNA in the lung cells and lung tissues in different stages of Bleomycin-A5-induced pulmonary fibrosis in rats were studied through Northern hybridization.
The expressions of TGF-beta 1 and PDGF mRNA reached their peaks in AMs of pulmonary fibrosis in rats on the 7th day after Bleomycin-A5 instillation. It was similar with that in the lung tissues. IGF-1 mRNA remained relatively stable in AMs during the course. PDGF and IGF-1 mRNA increased gradually in fibroblasts, and reached the highest expressions in the interstitial cells. There was almost no TGF-beta 1 mRNA expression in all groups of fibroblasts.
AMs are the main sources of TGF-beta 1 and PDGF in the lung tissues with fibrosis induced by Bleomycin-A5 AMs are activated in the first weekend and secrete TGF-beta 1 and PDGF to promote fibroblasts proliferation and fibrosis. As fibrosis developed, fibroblasts have established PDGF and IGF-1 autocrine and these three cytokines paracrine nets combined with the interstitial cells to promote lung fibrosis.
探讨肺泡巨噬细胞（AMs）、成纤维细胞及间质细胞在肺纤维化发生发展中的作用，以及转化生长因子-β1（TGF-β1）、血小板衍生生长因子（PDGF）和胰岛素样生长因子-1（IGF-1）之间的相互作用及其对肺纤维化的影响。
采用Northern杂交技术研究博来霉素A5诱导的大鼠肺纤维化不同阶段肺细胞及肺组织中TGF-β1、PDGF和IGF-1 mRNA的表达。
博来霉素A5滴注后第7天，大鼠肺纤维化肺泡巨噬细胞中TGF-β1和PDGF mRNA表达达到峰值，肺组织中情况与之相似。IGF-1 mRNA在整个过程中在肺泡巨噬细胞中相对稳定。PDGF和IGF-1 mRNA在成纤维细胞中逐渐增加，并在间质细胞中达到最高表达。各组成纤维细胞中几乎均无TGF-β1 mRNA表达。
在博来霉素A5诱导的肺纤维化组织中，肺泡巨噬细胞是TGF-β1和PDGF的主要来源，肺泡巨噬细胞在第1个周末被激活并分泌TGF-β1和PDGF，促进成纤维细胞增殖和纤维化。随着纤维化进展，成纤维细胞建立了PDGF和IGF-1自分泌，这三种细胞因子与间质细胞形成旁分泌网络，促进肺纤维化。
