Astashkin E I, Prikhod'ko A Z, Glezer S V, Shvarts G Ia, Krylov Iu F
Eksp Klin Farmakol. 1997 Mar-Apr;60(2):27-9.
It has been shown in vitro that sodium nitroprusside (SN) causes a concentration-dependent suppression of the enzymatic activity of aminopyridine demethylase-APDM(IC50 = 1.86 x 10(-4) M) and ethoxycoumarin O-deethylase-ECOD(IC50 = 1.46 x 10(-4) M) in hepatic microsomes of Wistar rats, which is related to different isoforms of cytochrome P-450. The inhibiting effect of other nitro compounds (nitroglycerin and isosorbide dinitrate) in relation to APDM is less marked than that of SN. These compounds have practically no effect on ECOD activity. It is assumed that the degree of the inhibiting effect of SN, nitroglycerin, and isosorbide dinitrate is connected with the mechanism of NO release.
体外实验表明，硝普钠（SN）可浓度依赖性抑制Wistar大鼠肝微粒体中氨基吡啶脱甲基酶 - APDM（IC50 = 1.86×10⁻⁴ M）和乙氧香豆素O - 脱乙基酶 - ECOD（IC50 = 1.46×10⁻⁴ M）的酶活性，这与细胞色素P - 450的不同同工型有关。其他硝基化合物（硝酸甘油和异山梨醇二硝酸酯）对APDM的抑制作用不如SN明显。这些化合物对ECOD活性几乎没有影响。据推测，SN、硝酸甘油和异山梨醇二硝酸酯的抑制作用程度与NO释放机制有关。
