在喹诺酮类药物存在的情况下，DNA 回旋酶可以切割短 DNA 片段。
DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.
作者信息
Cove M E, Tingey A P, Maxwell A
机构信息
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
出版信息
Nucleic Acids Res. 1997 Jul 15;25(14):2716-22. doi: 10.1093/nar/25.14.2716.
Abstract
We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a single-stranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can cleave duplexes down to approximately 20 bp in size in the presence of the quinolone drugs ciprofloxacin and oxolinic acid. Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases adjacent to the cleavage sites, whereas oxolinic acid stimulates cleavage predominantly at the previously determined site. With either drug, cleavage will not occur within 6 bases from the end of a DNA duplex or a nick. We suggest that cleavage site specificity with short DNA duplexes is determined by drug-DNA interactions whereas with longer fragments the positioning effect of the DNA wrap around gyrase prescribes the site of cleavage.
摘要
我们使用与含有首选促旋酶切割位点的单链M13衍生物退火的寡核苷酸，分析了促旋酶的DNA切割反应。我们发现，在喹诺酮类药物环丙沙星和恶喹酸存在的情况下，促旋酶能够切割大小约为20 bp的双链体。环丙沙星在其位点特异性上表现出变化，明显偏好切割位点相邻的G碱基，而恶喹酸主要刺激在先前确定的位点进行切割。使用任何一种药物时，在距DNA双链体末端或切口6个碱基范围内都不会发生切割。我们认为，短DNA双链体的切割位点特异性由药物与DNA的相互作用决定，而对于较长片段，DNA围绕促旋酶的定位效应规定了切割位点。
相似文献
1
DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.在喹诺酮类药物存在的情况下，DNA 回旋酶可以切割短 DNA 片段。
Nucleic Acids Res. 1997 Jul 15;25(14):2716-22. doi: 10.1093/nar/25.14.2716.
2
Site-specific interaction of DNA gyrase with DNA.DNA 回旋酶与 DNA 的位点特异性相互作用。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4165-9. doi: 10.1073/pnas.78.7.4165.
3
Sites of reaction of Escherichia coli DNA gyrase on pBR322 in vivo as revealed by oxolinic acid-induced plasmid linearization.通过恶喹酸诱导的质粒线性化揭示大肠杆菌DNA促旋酶在体内对pBR322的反应位点。
J Mol Biol. 1985 Jan 5;181(1):63-74. doi: 10.1016/0022-2836(85)90324-9.
4
DNA gyrase and topoisomerase IV on the bacterial chromosome: quinolone-induced DNA cleavage.细菌染色体上的DNA促旋酶和拓扑异构酶IV：喹诺酮诱导的DNA切割
J Mol Biol. 1996 May 17;258(4):627-37. doi: 10.1006/jmbi.1996.0274.
5
Sequence specificity of Bacillus subtilis DNA gyrase in vivo.枯草芽孢杆菌DNA促旋酶在体内的序列特异性
Genetica. 1991;85(1):3-12. doi: 10.1007/BF00056101.
6
DNA cleavage is not required for the binding of quinolone drugs to the DNA gyrase-DNA complex.喹诺酮类药物与DNA旋转酶-DNA复合物的结合并不需要DNA裂解。
Biochemistry. 1996 Jun 11;35(23):7387-93. doi: 10.1021/bi9603175.
7
The spacing of Escherichia coli DNA gyrase sites cleaved in vivo by treatment with oxolinic acid and sodium dodecyl sulfate.经恶喹酸和十二烷基硫酸钠处理后，在体内被切割的大肠杆菌DNA促旋酶位点的间距。
Biochimie. 1984 Nov-Dec;66(11-12):693-700. doi: 10.1016/0300-9084(84)90258-x.
8
gyrB-225, a mutation of DNA gyrase that compensates for topoisomerase I deficiency: investigation of its low activity and quinolone hypersensitivity.gyrB - 225，一种补偿拓扑异构酶I缺陷的DNA促旋酶突变：对其低活性和喹诺酮超敏反应的研究
J Mol Biol. 2001 Jun 22;309(5):1219-31. doi: 10.1006/jmbi.2001.4733.
9
In vivo cleavage of Escherichia coli BIME-2 repeats by DNA gyrase: genetic characterization of the target and identification of the cut site.DNA 回旋酶对大肠杆菌 BIME - 2 重复序列的体内切割：靶点的遗传特征及切割位点的鉴定
Mol Microbiol. 1997 Nov;26(4):767-77. doi: 10.1046/j.1365-2958.1997.6121983.x.
10
DNA gyrase on the bacterial chromosome. Oxolinic acid-induced DNA cleavage in the dnaA-gyrB region.细菌染色体上的DNA促旋酶。恶喹酸诱导的dnaA-gyrB区域的DNA切割。
J Mol Biol. 1988 May 5;201(1):229-33. doi: 10.1016/0022-2836(88)90449-4.
引用本文的文献
1
Structural basis of chiral wrap and T-segment capture by DNA gyrase.DNA 拓扑异构酶对手性包裹和 T 段捕获的结构基础。
Proc Natl Acad Sci U S A. 2024 Dec 3;121(49):e2407398121. doi: 10.1073/pnas.2407398121. Epub 2024 Nov 26.
2
The pentapeptide-repeat protein, MfpA, interacts with mycobacterial DNA gyrase as a DNA T-segment mimic.五肽重复蛋白 MfpA 作为 DNA T 段类似物与分枝杆菌 DNA 拓扑异构酶相互作用。
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). doi: 10.1073/pnas.2016705118.
3
The Microbial Toxin Microcin B17: Prospects for the Development of New Antibacterial Agents.微生物毒素微菌素 B17：开发新型抗菌剂的前景。
J Mol Biol. 2019 Aug 23;431(18):3400-3426. doi: 10.1016/j.jmb.2019.05.050. Epub 2019 Jun 8.
4
Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.利用细菌 DNA 拓扑异构酶作为药物靶点：现状与展望。
Appl Microbiol Biotechnol. 2011 Nov;92(3):479-97. doi: 10.1007/s00253-011-3557-z. Epub 2011 Sep 9.
5
A domain insertion in Escherichia coli GyrB adopts a novel fold that plays a critical role in gyrase function.大肠杆菌 GyrB 中的结构域插入采用了一种新的折叠方式，对拓扑异构酶功能起着至关重要的作用。
Nucleic Acids Res. 2010 Nov;38(21):7830-44. doi: 10.1093/nar/gkq665. Epub 2010 Jul 31.
6
Quinolone-DNA interaction: sequence-dependent binding to single-stranded DNA reflects the interaction within the gyrase-DNA complex.喹诺酮与DNA的相互作用：与单链DNA的序列依赖性结合反映了在回旋酶-DNA复合物中的相互作用。
Antimicrob Agents Chemother. 2003 Mar;47(3):854-62. doi: 10.1128/AAC.47.3.854-862.2003.
7
Importance of the fourth alpha-helix within the CAP homology domain of type II topoisomerase for DNA cleavage site recognition and quinolone action.II型拓扑异构酶CAP同源结构域内的第四个α-螺旋在DNA切割位点识别和喹诺酮作用中的重要性。
Antimicrob Agents Chemother. 2002 Sep;46(9):2735-46. doi: 10.1128/AAC.46.9.2735-2746.2002.
8
Quinolone-binding pocket of DNA gyrase: role of GyrB.DNA 回旋酶的喹诺酮结合口袋：GyrB 的作用
Antimicrob Agents Chemother. 2002 Jun;46(6):1805-15. doi: 10.1128/AAC.46.6.1805-1815.2002.
9
Replacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication.噬菌体Mu强解旋酶位点的替换及其对Mu DNA复制的影响。
J Bacteriol. 1999 Sep;181(18):5783-9. doi: 10.1128/JB.181.18.5783-5789.1999.
10
Sequence specific interaction of Mycobacterium smegmatis topoisomerase I with duplex DNA.耻垢分枝杆菌拓扑异构酶I与双链DNA的序列特异性相互作用。
Nucleic Acids Res. 1998 Apr 1;26(7):1668-74. doi: 10.1093/nar/26.7.1668.
本文引用的文献
1
DNA gyrase as a drug target.作为药物靶点的DNA促旋酶
Trends Microbiol. 1997 Mar;5(3):102-9. doi: 10.1016/S0966-842X(96)10085-8.
2
In the presence of subunit A inhibitors DNA gyrase cleaves DNA fragments as short as 20 bp at specific sites.在亚基A抑制剂存在的情况下，DNA促旋酶会在特定位点切割短至20个碱基对的DNA片段。
Nucleic Acids Res. 1997 Feb 1;25(3):604-11. doi: 10.1093/nar/25.3.604.
3
Appendix. II: Alignment of primary sequences of DNA topoisomerases.附录二：DNA拓扑异构酶一级序列比对
Adv Pharmacol. 1994;29B:271-97. doi: 10.1016/s1054-3589(08)61143-6.
4
Conversion of DNA gyrase into a conventional type II topoisomerase.DNA促旋酶向传统II型拓扑异构酶的转变。
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14416-21. doi: 10.1073/pnas.93.25.14416.
5
DNA topoisomerases.DNA拓扑异构酶
Annu Rev Biochem. 1996;65:635-92. doi: 10.1146/annurev.bi.65.070196.003223.
6
The interaction of coumarin antibiotics with fragments of DNA gyrase B protein.香豆素类抗生素与DNA促旋酶B蛋白片段的相互作用。
Biochemistry. 1996 Apr 16;35(15):5083-92. doi: 10.1021/bi952888n.
7
DNA cleavage is not required for the binding of quinolone drugs to the DNA gyrase-DNA complex.喹诺酮类药物与DNA旋转酶-DNA复合物的结合并不需要DNA裂解。
Biochemistry. 1996 Jun 11;35(23):7387-93. doi: 10.1021/bi9603175.
8
9
The 43-kilodalton N-terminal fragment of the DNA gyrase B protein hydrolyzes ATP and binds coumarin drugs.DNA 回旋酶 B 蛋白的 43 千道尔顿 N 端片段可水解 ATP 并结合香豆素类药物。
Biochemistry. 1993 Mar 16;32(10):2717-24. doi: 10.1021/bi00061a033.
10
The interaction between coumarin drugs and DNA gyrase.香豆素类药物与DNA回旋酶之间的相互作用。
Mol Microbiol. 1993 Aug;9(4):681-6. doi: 10.1111/j.1365-2958.1993.tb01728.x.
Zotero 插件