Alving B M, Evatt B L, Bell W R
Am J Physiol. 1977 Nov;233(5):562-7. doi: 10.1152/ajpheart.1977.233.5.H562.
The effect of intravenous infusions of thrombin on fibrinogen synthesis was evaluated in rabbits rendered afibrinogenemic by ancrod. Fibrinogen production and catabolism were measured simultaneously with 75Se-labeled selenomethionine (75SeM) and with 125I-labeled fibrinogen injected 5, 12, 18, or 26 h after ancrod. In normal rabbits the infusion of ancrod (2 U/kg body mass during 1 h) did not alter fibrinogen synthesis nor did it affect the rate of fibrinogen catabolism measured during a period of 5-120 h after ancrod administration. Normal rabbits that received purified bovine thrombin (100 U/kg body mass during 1 h) exhibited a twofold stimulation of fibrinogen synthesis as measured by the incorporation of 75SeM injected 5 h after thrombin. Rabbits made afibrinogenemic by infusion of ancrod 5 h before thrombin also showed a twofold increase in fibrinogen synthesis, as did animals that received ancrod immediately after thrombin. These experiments show that ancrod alone has no effect on fibrinogen production, and that thrombin stimulates fibrinogen synthesis in afibrinogenemic rabbits to the same extent as in controls.
在通过安克洛德使纤维蛋白原血症的兔子中，评估了静脉输注凝血酶对纤维蛋白原合成的影响。用75硒标记的硒代蛋氨酸（75SeM）以及在安克洛德给药后5、12、18或26小时注射的125碘标记的纤维蛋白原同时测量纤维蛋白原的产生和分解代谢。在正常兔子中，输注安克洛德（1小时内2单位/千克体重）既不改变纤维蛋白原合成，也不影响在安克洛德给药后5 - 120小时期间测量的纤维蛋白原分解代谢速率。接受纯化牛凝血酶（1小时内100单位/千克体重）的正常兔子，在凝血酶注射5小时后通过75SeM掺入测量显示纤维蛋白原合成有两倍的刺激。在凝血酶前5小时输注安克洛德使纤维蛋白原血症的兔子也显示纤维蛋白原合成增加两倍，与凝血酶后立即接受安克洛德的动物相同。这些实验表明，单独使用安克洛德对纤维蛋白原产生没有影响，并且凝血酶在纤维蛋白原血症兔子中刺激纤维蛋白原合成的程度与在对照中相同。
