硫胺素合成酶基因thiI的特性研究，thiI是鼠伤寒沙门氏菌中参与噻唑生物合成的一个新基因。
Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.
作者信息
Webb E, Claas K, Downs D M
机构信息
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
出版信息
J Bacteriol. 1997 Jul;179(13):4399-402. doi: 10.1128/jb.179.13.4399-4402.1997.
Abstract
Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling studies have demonstrated probable metabolic precursors to both. We present herein the initial characterization of thiI, a gene required for THZ synthesis. We show that thiI is a 1,449-bp open reading frame located at minute 9.65 on the S. typhimurium chromosome and that it encodes a 483-amino-acid protein with a predicted molecular mass of 55 kDa. Unlike genes in the thiamine biosynthetic operon at minute 90, thiI is not transcriptionally regulated by TPP.
摘要
硫胺素焦磷酸（TPP）是鼠伤寒沙门氏菌中必需的一种辅因子，它由4-氨基-5-羟甲基嘧啶（HMP）焦磷酸与4-甲基-5-（β-羟乙基）-噻唑（THZ）单磷酸缩合从头合成。THZ和HMP部分是独立合成的，标记研究已证明了两者可能的代谢前体。我们在此展示了thiI的初步特征，thiI是THZ合成所需的一个基因。我们表明，thiI是一个位于鼠伤寒沙门氏菌染色体9.65分钟处的1449碱基对的开放阅读框，它编码一个预测分子量为55 kDa的483个氨基酸的蛋白质。与位于90分钟处的硫胺素生物合成操纵子中的基因不同，thiI不受TPP的转录调控。
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2
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3
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Genetics. 1996 May;143(1):37-44. doi: 10.1093/genetics/143.1.37.
4
Involvement of the oxidative pentose phosphate pathway in thiamine biosynthesis in Salmonella typhimurium.氧化戊糖磷酸途径参与鼠伤寒沙门氏菌硫胺素的生物合成。
J Bacteriol. 1996 Mar;178(5):1476-9. doi: 10.1128/jb.178.5.1476-1479.1996.
5
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6
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7
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9
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10
apbA, a new genetic locus involved in thiamine biosynthesis in Salmonella typhimurium.apbA，一个参与鼠伤寒沙门氏菌硫胺素生物合成的新基因座。
J Bacteriol. 1994 Aug;176(16):4858-64. doi: 10.1128/jb.176.16.4858-4864.1994.
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