Yasui T, Takasugi N
Cell Tissue Res. 1977 Apr 29;179(4):475-82. doi: 10.1007/BF00219850.
Ovary-indepedent (estrogen-independent) irreversible proliferation and cornification on the vaginal epithelium in ovariectomized mice caused by neonatal injections of 20 microng estradiol-17 beta (E2) was suppressed by injections of 200 IU vitamin A acetate (VA) given simultaneously with E2. More than 2 months after the combined treatment were required for the suppression. A new type of cell of low electron density (Light cells) appeared at 40-120 days of age on the basal lamina of the vaginal epithelium in neonatally E2 plus VA-treated ovariectomized mice only at 50 days. Light cells contained small amounts of organelles. Columnar basal cells resembling those in normal estrous mice were also found in the E2 plus VA-treatedmice at 70-120 days. In one of the 120-day-old, E2 plus VA-treated mice, the epithelium consisted of only 2-3 layers of cells. Neither Light cells nor columnar cells were found in the atrophic epithelium.
新生期注射20微克17β-雌二醇（E2）导致去卵巢小鼠阴道上皮出现卵巢非依赖性（雌激素非依赖性）不可逆增殖和角质化，同时注射200国际单位醋酸维生素A（VA）可抑制这种现象。联合治疗后需要两个多月才能出现抑制效果。仅在出生后接受E2加VA处理的去卵巢小鼠50天时，在40至120日龄的阴道上皮基底层出现了一种新型低电子密度细胞（亮细胞）。亮细胞含有少量细胞器。在70至120日龄接受E2加VA处理的小鼠中也发现了类似于正常发情小鼠的柱状基底细胞。在一只120日龄接受E2加VA处理的小鼠中，上皮仅由2至3层细胞组成。在萎缩的上皮中未发现亮细胞和柱状细胞。
