Mak N K, Szeto Y Y, Fung M C, Leung K N, Kwan S K
Department of Biology, Hong Kong Baptist University, Hong Kong.
Chemotherapy. 1997 Jul-Aug;43(4):272-81. doi: 10.1159/000239578.
The effects of midazolam (MID) on the in vitro growth and differentiation of two murine myeloid leukemia WEHI 3B (JCS) and M1 cells were studied. MID inhibits the proliferation of both M1 and JCS cells in a dose-dependent manner. At the concentration of 10 micrograms/ml, MID was found to induce both monocytic and granulocytic differentiation of the JCS but not M1 cells. Induction of morphological differentiation of the JCS cells was also associated with the enhanced expression of the differentiation antigens Mac-1, F4/80, and Gr-1 for the cells. Results from mRNA phenotyping experiments also indicated that the expression of tumor necrosis factor (TNF-alpha) and neutrophil-specific J11d differentiation marker was significantly upregulated in MID-treated JCS cells. In addition, the phagocytic activity of MID-treated JCS cells was increased towards opsonized yeast cells. Results from this investigation suggested that MID may be used as an inducer for further study on the mechanisms of differentiation in these myeloid leukemia cells.
研究了咪达唑仑（MID）对两种小鼠髓系白血病细胞系WEHI 3B（JCS）和M1细胞体外生长及分化的影响。MID以剂量依赖性方式抑制M1和JCS细胞的增殖。在浓度为10微克/毫升时，发现MID可诱导JCS细胞发生单核细胞和粒细胞分化，但不能诱导M1细胞分化。JCS细胞形态分化的诱导还与细胞分化抗原Mac-1、F4/80和Gr-1表达增强有关。mRNA表型实验结果还表明，在经MID处理的JCS细胞中，肿瘤坏死因子（TNF-α）和中性粒细胞特异性J11d分化标志物的表达显著上调。此外，经MID处理的JCS细胞对调理酵母细胞的吞噬活性增强。本研究结果表明，MID可作为诱导剂，用于进一步研究这些髓系白血病细胞的分化机制。
