山羊对山羊关节炎-脑炎病毒的免疫反应。

Immune responses in goats to caprine arthritis-encephalitis virus.

作者信息

Ding E Y, Xiang W H

机构信息

Institute of Genetics, Fudan University, Shanghai, The People's Republic of China.

出版信息

Viral Immunol. 1997;10(2):111-5. doi: 10.1089/vim.1997.10.111.

DOI:10.1089/vim.1997.10.111

PMID:9210285

Abstract

In this study, goats were experimentally infected with caprine arthritis-encephalitis virus (CAEV). Anti-CAEV antibodies were detected in vivo using indirect enzyme-linked immunosorbent assay (ELISA) and Western blot assays. The results showed that the sensitivity of detection of anti-CAEV antibodies using indirect ELISA was relatively high, whereas Western blotting was very sensitive for detection of P28 anti-CAEV antibody as 4 days postinfection. Apparently, because of high immunogenicity of P28 antigen, the detection of antibodies specific for this viral protein may represent a valuable assay to detect early infection. CAEV related to other lentiviruses may be useful in studies of human immunodeficiency virus (HIV) and related viruses in animal models.

摘要

在本研究中，山羊被实验性感染山羊关节炎-脑炎病毒（CAEV）。使用间接酶联免疫吸附测定（ELISA）和蛋白质印迹法在体内检测抗CAEV抗体。结果表明，间接ELISA检测抗CAEV抗体的灵敏度相对较高，而蛋白质印迹法在感染后4天对检测P28抗CAEV抗体非常敏感。显然，由于P28抗原的高免疫原性，检测针对这种病毒蛋白的抗体可能是检测早期感染的一种有价值的测定方法。与其他慢病毒相关的CAEV可能在动物模型中用于人类免疫缺陷病毒（HIV）及相关病毒的研究。