Adebayo Isaac Abayomi, Walbey Dorothy, Adeyemo Oyewole
Department of Pathobiology, College of Veterinaiy Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, AL 36088, USA.
Cell Mol Biol (Noisy-le-grand). 2002;48 Online Pub:OL309-15.
The response of goats infected with caprine arthritis encephalitis virus was evaluated by estimating the binding antibodies in serum. The immunoreactive proteins of the CAEV, strain 63, were determined by immunobloting the lysate against the serum of the infected goat. The major proteins identified were of the molecular weights of 135, 120, 56, 44, 38, 28, 20 and 13 kDa. Proteins with less prominence were of the molecular weights of 96, 32 and 30 kDa. The responses to the viral proteins at several stages of the infection were examined. Antibodies to viral proteins of 135, 121 and 90 kDa were the first to appear in the goat kids that were apparently infected through breast-feeding by infected dam. The time of appearance of these antibodies in the serum varied widely between the goats from one to six months, and preceded the appearence of antibodies to other proteins by two to four weeks. Salient variations revealed in the immunoblot patterns reflect the differences in the immunological responses by the goats to the viral proteins. Littermate goat kids infected through the same dam showed slightly different responses. In the same manner, dissimilarities were observed in the patterns produced by the serums of the damand the corresponding offsprings, when run against the same virus lysate. This study indicates that some differences occur in the immunoblot pattern when this assay is used for diagnosis, although such discrepancies should not affect the interpretation of the diagnostic assay. The host differences in the response to the viral proteins also suggest the potential problems that can be encountered developing an effective vaccine for CAEV, if the binding antibody production reflects that of the neutralizing antibodies in the goats. For effective vaccine coverage, there should be little variability in the immunogenicity of candidate vaccine antigens among the hosts.
通过评估血清中的结合抗体来评价感染山羊关节炎脑炎病毒的山羊的反应。用感染山羊的血清对CAEV 63株裂解物进行免疫印迹，确定其免疫反应蛋白。鉴定出的主要蛋白分子量为135、120、56、44、38、28、20和13 kDa。不太突出的蛋白分子量为96、32和30 kDa。检测了感染几个阶段对病毒蛋白的反应。135、121和90 kDa病毒蛋白的抗体最早出现在显然通过感染母羊母乳喂养而感染的山羊羔中。这些抗体在血清中出现的时间在山羊之间差异很大，为1至6个月，比其他蛋白抗体的出现提前2至4周。免疫印迹模式中显示的显著差异反映了山羊对病毒蛋白免疫反应的差异。通过同一母羊感染的同窝山羊羔表现出略有不同的反应。同样，当用相同的病毒裂解物检测时，母羊和相应后代血清产生的模式也存在差异。本研究表明，当该检测方法用于诊断时，免疫印迹模式会出现一些差异，尽管这些差异不应影响诊断检测结果的解读。宿主对病毒蛋白反应的差异也表明，如果结合抗体的产生反映了山羊体内中和抗体的产生，那么在开发针对CAEV的有效疫苗时可能会遇到潜在问题。为了实现有效的疫苗覆盖，候选疫苗抗原在宿主中的免疫原性应几乎没有变异性。
