大肠杆菌单链DNA结合蛋白和哺乳动物复制蛋白A的结合位点大小分别为65个核苷酸和≥54个核苷酸。
The binding-site sizes of Escherichia coli single-stranded-DNA-binding protein and mammalian replication protein A are 65 and >/= 54 nucleotides respectively.
作者信息
Mitas M, Chock J Y, Christy M
机构信息
Department of Biochemistry and Molecular Biology, 246 Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA.
出版信息
Biochem J. 1997 Jun 15;324 ( Pt 3)(Pt 3):957-61. doi: 10.1042/bj3240957.
Abstract
The electrophoretic mobilities of complexes formed with single-stranded (ss) DNA and tetrameric Escherichia coli ssDNA-binding protein (EcoSSB) or mammalian replication protein A (RPA) were analysed. The electrophoretic mobilities of the complexes in a native polyacrylamide gel increased as the lengths of the DNA increased from 28 to 70 nt, thus revealing paradoxical 'descending-staircase' patterns. Increases in the electrophoretic mobilities of EcoSSB.ssDNA complexes were observed when the lengths of the bound DNA were increased by 1 nt. Quantitative analyses of the complexes suggested that the binding-sites sizes of EcoSSB and RPA were 65 and >=54 nt respectively. The binding-site size for RPA is at least 24 nt larger than previously reported.
摘要
分析了与单链（ss）DNA和四聚体大肠杆菌ssDNA结合蛋白（EcoSSB）或哺乳动物复制蛋白A（RPA）形成的复合物的电泳迁移率。在天然聚丙烯酰胺凝胶中，随着DNA长度从28 nt增加到70 nt，复合物的电泳迁移率增加，从而揭示出矛盾的“下降阶梯”模式。当结合的DNA长度增加1 nt时，观察到EcoSSB.ssDNA复合物的电泳迁移率增加。对复合物的定量分析表明，EcoSSB和RPA的结合位点大小分别为65 nt和>=54 nt。RPA的结合位点大小比先前报道的至少大24 nt。
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