Stead D A, Richards R M
School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen, UK.
J Chromatogr B Biomed Sci Appl. 1997 Jun 6;693(2):415-21. doi: 10.1016/s0378-4347(97)00032-7.
Following the development of a sensitive high-performance liquid chromatographic (HPLC) assay for gentamicin in biological matrices, the utility of this assay for the determination of other clinically important aminoglycosides (neomycin, netilmicin and sisomicin) in bacterial culture media or plasma is demonstrated. The high sensitivity of the assay enables direct measurement of the aminoglycoside content of bacterial cells cultured in the presence of unlabelled drug.
在开发出一种用于生物基质中庆大霉素的灵敏高效液相色谱（HPLC）测定法之后，证明了该测定法在测定细菌培养基或血浆中其他临床上重要的氨基糖苷类药物（新霉素、奈替米星和西索米星）方面的实用性。该测定法的高灵敏度能够直接测量在未标记药物存在下培养的细菌细胞中的氨基糖苷类药物含量。
