Taliani G, Celestino D, Badolato M C, Pennica A, Bozza A, Poliandri G, Riccieri V, Benfari G, Sebastiani A, De Bac C, Quaranta G, Aceti A
Department of Infectious and Tropical Diseases, La Sapienza University, Rome, Italy.
J Hepatol. 1997 Jun;26(6):1200-6. doi: 10.1016/s0168-8278(97)80452-7.
Hepatitis C virus genome (HCV-RNA) has been detected in whole salivary gland tissue of chronically infected patients. However, contamination of the tissue by plasma or blood cells was not excluded by the previous reports.
To assess whether HCV infects the salivary gland epithelial cells in patients with chronic HCV liver disease.
Twenty unselected patients with chronic active hepatitis (11 cases) or active cirrhosis (nine cases) were examined. Serum and saliva samples were obtained from all patients, 12 of whom (seven, chronic active hepatitis; five, active cirrhosis) underwent salivary gland biopsy. PCR for HCV-RNA was performed on RNA extracted from serum, saliva and salivary gland epithelial cells collected by isokinetic gradient separation after trypsin digestion of whole salivary gland tissue. Saliva samples were also examined for the presence of secretory IgA anti-HCV by gel chromatography and ELISA testing.
HCV-RNA was detected in all sera with titers ranging from 5.42 x 10(5) genome equivalents/ml to 123.2 x 10(5) genome equivalents/ml. Thirteen patients were infected with genotype 1b, four patients had genotype 1a, two patients had genotype 2a and one patient was unclassifiable. Low titer HCV-RNA (<2 x 10(5) genome equivalents/ml) was detected in 3/20 saliva samples (15%) from highly viremic patients infected with 1b genotype. RNA extracted from salivary gland epithelial cells consistently tested negative for HCV-RNA. In addition, all saliva specimens tested negative for secretory-IgA (S-IgA) anti-HCV, even after a 10-fold concentration of the samples.
There was no evidence that HCV infects the salivary gland epithelial cells in our viremic patients with HCV chronic liver disease. Low level HCV-RNA in saliva is most probably due to virus spillover from blood.
在慢性感染患者的整个唾液腺组织中已检测到丙型肝炎病毒基因组（HCV-RNA）。然而，先前的报告并未排除组织被血浆或血细胞污染的可能性。
评估丙型肝炎病毒是否感染慢性丙型肝炎肝病患者的唾液腺上皮细胞。
对20例未经挑选的慢性活动性肝炎患者（11例）或活动性肝硬化患者（9例）进行检查。采集所有患者的血清和唾液样本，其中12例患者（7例慢性活动性肝炎；5例活动性肝硬化）接受了唾液腺活检。对经胰蛋白酶消化全唾液腺组织后通过等速梯度分离收集的血清、唾液和唾液腺上皮细胞中提取的RNA进行HCV-RNA的PCR检测。还通过凝胶色谱法和ELISA检测唾液样本中分泌型IgA抗HCV的存在情况。
所有血清中均检测到HCV-RNA，滴度范围为5.42×10⁵基因组当量/毫升至123.2×10⁵基因组当量/毫升。13例患者感染1b基因型，4例患者为1a基因型，2例患者为2a基因型，1例患者无法分型。在感染1b基因型的高病毒血症患者的20份唾液样本中有3份（15%）检测到低滴度HCV-RNA（<2×10⁵基因组当量/毫升）。从唾液腺上皮细胞中提取的RNA HCV-RNA检测结果始终为阴性。此外，即使样本浓缩10倍，所有唾液标本的分泌型IgA（S-IgA）抗HCV检测结果均为阴性。
没有证据表明丙型肝炎病毒感染了我们患有慢性丙型肝炎肝病的病毒血症患者的唾液腺上皮细胞。唾液中低水平的HCV-RNA很可能是由于病毒从血液中溢出所致。
