Caffrey C R, Ruppel A
Institut für Tropenhygiene, Heidelberg, Germany.
Parasitol Res. 1997;83(6):632-5. doi: 10.1007/s004360050310.
Both cathepsin B-like and cathepsin L-like endopeptidase activities have been described in schistosomes, but their relative contribution to proteinolysis remains controversial. In an attempt to clarify which type of activity predominates, the selective mammalian cathepsin B inhibitor CA-074 was tested under standardized assay conditions with different preparations from Schistosoma mansoni and S. japonicum. CA-074 (0.94 microM) inhibited at least 92% and 80% of proteinolytic activity, respectively, for these species: completely inhibited bovine-spleen cathepsin B activity; but showed only marginal inhibition (4%) of rat-liver cathepsin L activity. We discuss the results with respect to previous studies and conclude that schistosome cathepsin B-like, not L-like, activity predominates.
在血吸虫中已发现组织蛋白酶B样和组织蛋白酶L样内肽酶活性，但它们对蛋白水解的相对作用仍存在争议。为了明确哪种类型的活性占主导，在标准化测定条件下，用来自曼氏血吸虫和日本血吸虫的不同制剂测试了选择性哺乳动物组织蛋白酶B抑制剂CA-074。对于这些物种，CA-074（0.94微摩尔）分别抑制了至少92%和80%的蛋白水解活性：完全抑制了牛脾组织蛋白酶B的活性；但对大鼠肝脏组织蛋白酶L的活性仅表现出轻微抑制（4%）。我们结合先前的研究讨论了这些结果，并得出结论，血吸虫中占主导的是组织蛋白酶B样活性，而非组织蛋白酶L样活性。
