聚合酶链式反应介导的嗜酸、生物浸出相关细菌的检测
PCR-mediated detection of acidophilic, bioleaching-associated bacteria.
作者信息
De Wulf-Durand P, Bryant L J, Sly L I
机构信息
Department of Microbiology, University of Queensland, Brisbane, Australia.
出版信息
Appl Environ Microbiol. 1997 Jul;63(7):2944-8. doi: 10.1128/aem.63.7.2944-2948.1997.
Abstract
The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.
摘要
通过培养方法从采矿环境中检测嗜酸微生物既耗时又不可靠。人们开发了几种聚合酶链式反应（PCR）方法，用于从与酸性采矿环境相关的六种细菌系统型的DNA中扩增小亚基核糖体RNA（rRNA）序列，从而能够检测低至10飞克浓度的目标DNA。
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8
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