Xia Lexian, Dai Songlin, Yin Chu, Hu Yuehua, Liu Jianshe, Qiu Guanzhou
Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Resources Processing and Bioengineering, Central South University, Changsha, People's Republic of China.
J Ind Microbiol Biotechnol. 2009 Jun;36(6):845-51. doi: 10.1007/s10295-009-0560-9. Epub 2009 Mar 31.
Two sphalerite samples with different iron/sulphur (Fe/S) ratios, Shuikousan ore (Fe/S 0.2) and Dachang ore (Fe/S 0.52), were processed using three microbial species, Leptospirillum ferriphilum, Acidithiobacillus ferrooxidans and Acidithiobacillus caldus. Following 20 days of bioleaching in shake flask cultures, a higher zinc (Zn) extraction (96%) was achieved with Shuikousan ore than with Dachange ore (72%). The extraction efficiency increased when elemental S was added to Dachang ore to attain the same Fe/S ratio as that for Shuikousan ore. Following the addition of S, the redox potential, pH and total dissolved Fe for Dachang ore demonstrated similar behaviors to those of Shuikousan ore. Acidithiobacillus caldus and L. ferriphilum became the dominant species during the bioleaching of sphalerite with a high Fe/S ratio. In contrast, the dominant species were A. ferrooxidans and A. caldus during the bioleaching of sphalerite with a low Fe/S ratio. These results show that the Fe/S ratio has a significant influence on the bioleaching behavior of sphalerite and the composition of the microbial community.
采用嗜铁钩端螺旋菌、氧化亚铁硫杆菌和嗜热嗜酸硫杆菌这三种微生物，对两种铁/硫（Fe/S）比例不同的闪锌矿样品——水口山矿石（Fe/S 0.2）和大厂矿石（Fe/S 0.52）进行了处理。在摇瓶培养中进行20天的生物浸出后，水口山矿石的锌（Zn）提取率（96%）高于大厂矿石（72%）。当向大厂矿石中添加元素硫以使其Fe/S比例与水口山矿石相同时，提取效率有所提高。添加硫后，大厂矿石的氧化还原电位、pH值和总溶解铁表现出与水口山矿石相似的行为。在高Fe/S比例闪锌矿的生物浸出过程中，嗜热嗜酸硫杆菌和嗜铁钩端螺旋菌成为优势菌种。相比之下，在低Fe/S比例闪锌矿的生物浸出过程中，优势菌种为氧化亚铁硫杆菌和嗜热嗜酸硫杆菌。这些结果表明，Fe/S比例对闪锌矿的生物浸出行为和微生物群落组成有显著影响。
