多种温和嗜热硫杆菌血清型，生物浸矿微生物免疫检测法的局限性。
Multiple Serotypes of the Moderate Thermophile Thiobacillus caldus, a Limitation of Immunological Assays for Biomining Microorganisms.
出版信息
Appl Environ Microbiol. 1996 Nov;62(11):4243-6. doi: 10.1128/aem.62.11.4243-4246.1996.
Abstract
Phylogenetic and phenotypic analysis indicates that a moderately thermophilic isolate, C-SH12, from Australia belongs to the species Thiobacillus caldus. Antiserum generated against whole cells of T. caldus KU recognized protein antigens common to cell lysates of the three T. caldus strains KU, BC13, and C-SH12 but did not recognize whole cells of isolate C-SH12. Differences in the lipopolysaccharide (LPS) of strain C-SH12 and those of the other two T. caldus strains were found, and the anti-KU antiserum did not recognize the LPS from strain C-SH12. These data indicate that this T. caldus isolate belongs to a serotype different from that of strains KU and BC13.
摘要
系统发育和表型分析表明，来自澳大利亚的中度嗜热分离株 C-SH12 属于硫杆菌属的 T. caldus 物种。针对 T. caldus KU 全细胞产生的抗血清识别来自三个 T. caldus 菌株 KU、BC13 和 C-SH12 的细胞裂解物中的共同蛋白抗原，但不识别分离株 C-SH12 的全细胞。发现菌株 C-SH12 的脂多糖 (LPS) 与其他两个 T. caldus 菌株的 LPS 存在差异，并且抗 KU 血清不识别来自菌株 C-SH12 的 LPS。这些数据表明，这种 T. caldus 分离株属于不同于 KU 和 BC13 菌株的血清型。
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