Nagata S, Yamamoto K, Ueno Y, Kurata T, Chiba J
Department of Pathology, National Institute of Health, Tokyo, Japan.
Hybridoma. 1991 Jun;10(3):369-78. doi: 10.1089/hyb.1991.10.369.
Vesicular stomatitis virus (VSV)-mediated cell fusion was recently proposed as an alternative fusion technique to generate monoclonal antibody (MAb)-producing hybridomas. In order to further examine this technique, we made direct comparative experiments among VSV, Sendai virus (SV) and polyethylene glycol (PEG)-mediated cell fusion in generating MAb-producing hybridomas. The distribution of immunoglobulin (Ig) isotypes secreted by the hybridomas obtained, as well as hybridoma yield and specific hybridoma yield, was compared. The results show that VSV-fusion yielded almost the same number of specific hybridomas as SV- and PEG-fusion in spite of its lower fusion frequency. In addition, VSV-fusion preferentially gave Ig-producing, especially IgG-producing, hybridomas. SV-fusion yielded both hybridomas and specific hybridomas with similar frequency to PEG-fusion, but IgM-producers predominated. These results demonstrate that fusion method has a considerable influence on the isotypes of obtained antibodies, and also suggest an advantage of VSV-fusion for production of IgG monoclonal antibodies.
水泡性口炎病毒（VSV）介导的细胞融合最近被提议作为一种替代融合技术，用于产生分泌单克隆抗体（MAb）的杂交瘤。为了进一步研究该技术，我们对VSV、仙台病毒（SV）和聚乙二醇（PEG）介导的细胞融合在产生分泌MAb的杂交瘤方面进行了直接对比实验。比较了所获得的杂交瘤分泌的免疫球蛋白（Ig）同种型分布，以及杂交瘤产量和特异性杂交瘤产量。结果表明，尽管VSV融合的融合频率较低，但产生的特异性杂交瘤数量与SV和PEG融合几乎相同。此外，VSV融合优先产生分泌Ig的杂交瘤，尤其是分泌IgG的杂交瘤。SV融合产生杂交瘤和特异性杂交瘤的频率与PEG融合相似，但以分泌IgM的杂交瘤为主。这些结果表明融合方法对所获得抗体的同种型有相当大的影响，也提示了VSV融合在产生IgG单克隆抗体方面的优势。
