Van Driel B E, Lyon H, Hoogenraad D C, Anten S, Hansen U, Van Noorden C J
Academic Medical Center, University of Amsterdam, Laboratory of Cell Biology and Histology, The Netherlands.
Free Radic Biol Med. 1997;23(3):435-44. doi: 10.1016/s0891-5849(97)00102-0.
Decreased intracellular SOD protein levels and activity have been related with malignancy in the past. To investigate their relevance in the carcinogenetic process in the colon, we determined quantitatively CuZn-SOD and Mn-SOD levels and total SOD activity by histochemical means in human normal colorectal mucosa, adenomas, and carcinomas. Protein levels and activity were significantly decreased in carcinomas. CuZn-SOD protein levels, but not Mn-SOD levels or total SOD activity were related with differentiation grade and to a lesser extent with Dukes stage. Moderately differentiated carcinomas and Dukes stage A carcinomas showed lowest levels. Some carcinomas expressed elevated levels of CuZn-SOD and this was an indication of poor survival. It is concluded that decreased SOD expression is not a prognostic marker and seems to be a secondary phenomenon rather than directly linked with the carcinogenetic process.
过去，细胞内超氧化物歧化酶（SOD）蛋白水平和活性的降低与恶性肿瘤有关。为了研究它们在结肠癌发生过程中的相关性，我们通过组织化学方法定量测定了人类正常结直肠黏膜、腺瘤和癌组织中铜锌超氧化物歧化酶（CuZn-SOD）和锰超氧化物歧化酶（Mn-SOD）的水平以及总超氧化物歧化酶活性。癌组织中的蛋白水平和活性显著降低。CuZn-SOD蛋白水平与分化程度相关，与Dukes分期在较小程度上相关，而Mn-SOD水平或总超氧化物歧化酶活性与分化程度及Dukes分期无关。中度分化癌和Dukes A期癌的CuZn-SOD蛋白水平最低。一些癌组织中CuZn-SOD表达水平升高，这表明患者预后较差。研究得出结论，超氧化物歧化酶表达降低不是一个预后标志物，似乎是一种继发现象，而非直接与致癌过程相关。
