Nozoe Tadahiro, Honda Masayuki, Inutsuka Sadaaki, Yasuda Mitshuhiro, Korenaga Daisuke
Department of General Surgery, Fukuoka Dental College Hospital, Japan.
Oncol Rep. 2003 Jan-Feb;10(1):39-43.
Manganese superoxide dismutase (MnSOD) is an important superoxide anion scavenger located in mitochondria and protects cells from the damage caused by reactive oxygen species. The oxygen balance has been reported to be related to the occurrence and invasiveness of malignant tumors. The aim of the current study was to elucidate the clinicopathological significance of immunohistochemical MnSOD expression in colorectal carcinoma. Consecutive 186 primary colorectal carcinomas which had been resected from 1987 to 1999 were examined for MnSOD expression by means of an immunohistochemical investigation. Sixty nine carcinomas (37.1%) had a positive response to MnSOD. The proportion of lymph node metastasis in MnSOD positive carcinomas (49.3%, 34 out of 69) was significantly higher than that in MnSOD negative carcinomas (26.5%, 31 out of 117; p=0.002). The proportion of patients classified to Dukes' stage A, B, C, and D in MnSOD positive carcinomas was 10.1, 33.3, 40.7, and 15.9%, respectively, and those were 23.1, 49.5, 24.8, and 2.6%, respectively in MnSOD negative carcinomas (p=0.0001). The survival rate in patients with MnSOD positive carcinomas was significantly worse than that in patients with MnSOD negative carcinomas (p=0.01). Multivariate analysis demonstrated that Dukes' stage of tumors was found to be a factor independently associated with prognosis of the patients. Our results showed that immunohistochemical expression of MnSOD can give auxiliary clinical information for malignant potential of colorectal carcinoma.
锰超氧化物歧化酶（MnSOD）是一种位于线粒体中的重要超氧阴离子清除剂，可保护细胞免受活性氧物质造成的损伤。据报道，氧平衡与恶性肿瘤的发生和侵袭性有关。本研究的目的是阐明免疫组化检测MnSOD在结直肠癌中的表达的临床病理意义。通过免疫组化研究，对1987年至1999年间连续切除的186例原发性结直肠癌进行MnSOD表达检测。69例癌（37.1%）对MnSOD呈阳性反应。MnSOD阳性癌的淋巴结转移率（49.3%，69例中的34例）显著高于MnSOD阴性癌（26.5%，117例中的31例；p = 0.002）。MnSOD阳性癌中归类为Dukes分期A、B、C和D期的患者比例分别为10.1%、33.3%、40.7%和15.9%，而MnSOD阴性癌中分别为23.1%、49.5%、24.8%和2.6%（p = 0.0001）。MnSOD阳性癌患者的生存率显著低于MnSOD阴性癌患者（p = 0.01）。多因素分析表明，肿瘤的Dukes分期是与患者预后独立相关的因素。我们的结果表明，MnSOD的免疫组化表达可为结直肠癌的恶性潜能提供辅助临床信息。
