追踪于DNA上的转录激活因子的双重靶标。
Dual targets of a transcriptional activator that tracks on DNA.
作者信息
Sanders G M, Kassavetis G A, Geiduschek E P
机构信息
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0634, USA.
出版信息
EMBO J. 1997 Jun 2;16(11):3124-32. doi: 10.1093/emboj/16.11.3124.
Abstract
The sliding clamp of the bacteriophage T4 DNA polymerase, gp45, is also the proximal effector for activation of transcription of T4 late genes. We have identified the phage T4-encoded sigma factor gp55 and the co-activator gp33 as targets of gp45 in promoter complexes, and have shown that a conserved carboxy-terminal amino acid sequence of gp55 and gp33 is required for interaction with gp45. The respective contribution of each target-gp45 interaction to activation of transcription has been assessed by measuring promoter opening rates. The opening rate supported by interaction with both targets is far greater than the arithmetical sum of the separate contributions of each target, implying a synergistic activation of transcription through at least two separate interactions of the trimeric gp45.
摘要
噬菌体T4 DNA聚合酶的滑动夹蛋白gp45，也是T4晚期基因转录激活的近端效应物。我们已确定噬菌体T4编码的σ因子gp55和共激活因子gp33是启动子复合物中gp45的作用靶点，并表明gp55和gp33保守的羧基末端氨基酸序列是与gp45相互作用所必需的。通过测量启动子开放速率，评估了每个靶点与gp45相互作用对转录激活的各自贡献。与两个靶点相互作用所支持的开放速率远大于每个靶点单独贡献的算术和，这意味着通过三聚体gp45的至少两种独立相互作用对转录进行协同激活。
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