Coleman J L, Gebbia J A, Piesman J, Degen J L, Bugge T H, Benach J L
State of New York Department of Health, Health Sciences Center, State University of New York at Stony Brook, 11794, USA.
Cell. 1997 Jun 27;89(7):1111-9. doi: 10.1016/s0092-8674(00)80298-6.
The role of the host plasminogen activation system in transmission of and invasion by Borrelia burgdorferi, the tick-borne spirochetal agent of Lyme disease, was investigated using plasminogen (Plg)-knockout mice. PLG was not detected in spirochetes from unfed ticks, but binding occurred as ticks fed on the host's blood. Plasminogen activators were derived from the host blood meal. PLG was required for efficient dissemination of B. burgdorferi within the tick and for enhancement of spirochetemia in mice but was not critical for transmission and infection. These results provide evidence for a bacterium using a vertebrate protease to disseminate in an invertebrate vector and underscores the interplay among vector, pathogen, and host in promoting the life cycle and disease.
利用纤溶酶原（Plg）基因敲除小鼠，研究了宿主纤溶酶原激活系统在莱姆病的蜱传螺旋体病原体——伯氏疏螺旋体传播和侵袭中的作用。在未进食的蜱的螺旋体中未检测到PLG，但随着蜱吸食宿主血液，两者发生了结合。纤溶酶原激活剂来自宿主血餐。PLG对于伯氏疏螺旋体在蜱体内的有效传播以及小鼠螺旋体血症的增强是必需的，但对于传播和感染并非至关重要。这些结果为一种细菌利用脊椎动物蛋白酶在无脊椎动物载体中传播提供了证据，并强调了载体、病原体和宿主之间在促进生命周期和疾病方面的相互作用。
