Mascarenhas J D, Gusmão R H, Gabbay Y B, Monteiro T A, Gomes J B, Linhares A C
Serviço de Virologia Geral, Instituto Evandro Changas, Belém, Pará, Brasil.
Rev Inst Med Trop Sao Paulo. 1996 Jul-Aug;38(4):249-52. doi: 10.1590/s0036-46651996000400002.
Concomitant serotypes 1 and 4 infections were detected in a 15-month old female child with community-acquired diarrhoea which lasted 7 days and coursed with moderate dehydration. The evidence for dual rotavirus infection was offered by the following findings: a) enzyme-linked immunosorbent assay (ELISA) positive reactions to both 1 and 4 serotypes; and b) extra-migrating bands at electrophoresis of RNA in polyacrylamide gel (PAGE). These results suggest that children living under poor sanitation conditions are heavily exposed to rotavirus infections; in addition, the co-circulation of different serotypes in the same setting sustains the current concept that a rotavirus vaccine should be multivalent, in order to protect children against the four epidemiologically important rotavirus G serotypes.
在一名15个月大的社区获得性腹泻女童中检测到1型和4型轮状病毒的合并感染，腹泻持续7天，并伴有中度脱水。以下发现提供了双重轮状病毒感染的证据：a）酶联免疫吸附测定（ELISA）对1型和4型血清型均呈阳性反应；b）聚丙烯酰胺凝胶电泳（PAGE）中RNA出现额外迁移条带。这些结果表明，生活在卫生条件差的环境中的儿童极易感染轮状病毒；此外，在同一环境中不同血清型的共同流行支持了目前的观点，即轮状病毒疫苗应为多价疫苗，以保护儿童免受四种在流行病学上重要的轮状病毒G血清型的感染。
