Dancoine F, Couplet G, Buvat J, Guittard C, Marcolin G, Fourlinnie J C
Laboratoire de Biologie Médicale Drs Leduc et Ass, Lille, France.
Clin Chem. 1997 Jul;43(7):1165-71.
We examined an immunoassay for estradiol (E2) on the Immulite-an automated, random access chemiluminescent immunoassay system-to determine its accuracy and precision required for in vitro fertilization (IVF) studies. The assay, which has a reportable range from 73 to 7300 pmol/L, demonstrated good linearity under dilution, a detection limit of 44 pmol/L, and interassay CVs of 12.6% and 7.6% at 466 and 6164 pmol/L, respectively. In a retrospective analysis of 545 serum samples, the assay showed adequate agreement with an antibody-coated-tube RIA. The two E2 assays showed good agreement, even on samples from patients receiving a variety of different estrogen replacement therapies. Longitudinal studies of individual IVF cycles showed good parallelism between the automated system and the RIA, and results by the automated assay correlated well with the total number of follicles.
我们在Immulite（一种自动化随机进样化学发光免疫分析系统）上检测了雌二醇（E2）免疫分析方法，以确定其在体外受精（IVF）研究中所需的准确性和精密度。该分析方法的报告范围为73至7300 pmol/L，在稀释条件下显示出良好的线性，检测限为44 pmol/L，在466和6164 pmol/L时批间变异系数分别为12.6%和7.6%。在对545份血清样本的回顾性分析中，该分析方法与抗体包被管放射免疫分析（RIA）显示出充分的一致性。两种E2分析方法显示出良好的一致性，即使在接受各种不同雌激素替代疗法的患者样本上也是如此。对单个IVF周期的纵向研究表明，自动化系统和RIA之间具有良好的平行性，自动化分析结果与卵泡总数具有良好的相关性。
