Satoh K, Sakagami H
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Anticancer Res. 1997 May-Jun;17(3C):2175-9.
The effect of twenty amino acids on the radical intensity of four antioxidants (sodium L-ascorbate, sodium 5,6-benzylidene-L-ascorbate, gallic acid, caffeic acid) was investigated, using ESR spectroscopy. Methionine and methional did not significantly affect the radical intensity of these antioxidants. Methionine sulfoxide slightly enhanced the radical intensity of sodium ascorbate and sodium 5,6-benzylidene-L-ascorbate, but did not that of gallic acid and caffeic acid. Cysteine, N-acetyl cysteine and glutathione significantly reduced the radical intensity and cytotoxic activity of these antioxidants except for sodium 5,6-benzylidene-L-ascorbate. The other amino acids were inactive. The present study further supports that these antioxidants induce cytotoxicity via their pro-oxidant action.
采用电子自旋共振光谱法研究了20种氨基酸对4种抗氧化剂（L-抗坏血酸钠、5,6-亚苄基-L-抗坏血酸钠、没食子酸、咖啡酸）自由基强度的影响。蛋氨酸和甲硫醛对这些抗氧化剂的自由基强度没有显著影响。甲硫氨酸亚砜略微增强了抗坏血酸钠和5,6-亚苄基-L-抗坏血酸钠的自由基强度，但对没食子酸和咖啡酸没有影响。半胱氨酸、N-乙酰半胱氨酸和谷胱甘肽显著降低了除5,6-亚苄基-L-抗坏血酸钠外的这些抗氧化剂的自由基强度和细胞毒性活性。其他氨基酸没有活性。本研究进一步支持这些抗氧化剂通过其促氧化作用诱导细胞毒性。
