Fuller M E, Manning J F
Environmental Research Division, ER/203-J070, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA.
Curr Microbiol. 1997 Aug;35(2):77-83. doi: 10.1007/s002849900216.
A systematic evaluation of the ability of different bacterial genera to transform 2,4,6-trinitrotoluene (TNT), and grow in its presence, was conducted. Aerobic Gram-negative organisms degraded TNT and evidenced net consumption of reduced metabolites when cultured in molasses medium. Some Gram-negative isolates transformed all the initial TNT to undetectable metabolites, with no adsorption of TNT or metabolites to cells. Growth and TNT transformation capacity of Gram-positive bacteria both exhibited 50% reductions in the presence of approximately 10 microg TNT ml-1. Most non-sporeforming Gram-positive organisms incubated in molasses media amended with 80 microg TNT ml-1 became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 microg TNT ml-1, indicating that TNT sensitivity is linked to metabolic activity. These results indicate that the microbial ecology of soil may be severely impacted by TNT contamination.
我们对不同细菌属转化2,4,6-三硝基甲苯（TNT）并在其存在下生长的能力进行了系统评估。需氧革兰氏阴性菌在糖蜜培养基中培养时可降解TNT，并显示出还原代谢产物的净消耗。一些革兰氏阴性分离株将所有初始TNT转化为无法检测到的代谢产物，且TNT或代谢产物均未吸附到细胞上。在约10微克TNT/毫升的存在下，革兰氏阳性菌的生长和TNT转化能力均降低了50%。大多数在添加80微克TNT/毫升的糖蜜培养基中培养的非芽孢形成革兰氏阳性菌无法培养，而在添加98微克TNT/毫升的矿物培养基中培养时，所有测试菌株仍可培养，这表明TNT敏感性与代谢活性有关。这些结果表明，TNT污染可能会严重影响土壤的微生物生态。
