Sen C K, Atalay M, Agren J, Laaksonen D E, Roy S, Hänninen O
Department of Physiology, Faculty of Medicine, University of Kuopio, 70211 Kuopio, Finland.
J Appl Physiol (1985). 1997 Jul;83(1):189-95. doi: 10.1152/jappl.1997.83.1.189.
Fish oil supplementation and physical exercise may induce oxidative stress. We tested the effects of 8 wk of alpha-tocopherol (vitamin E) and fish oil (FO) supplementation on resting and exercise-induced oxidative stress. Rats (n = 80) were divided into groups supplemented with FO, FO and vitamin E (FOVE), soy oil (SO), and SO and vitamin E (SOVE), and for FOVE and SOVE they were divided into corresponding exercise groups (FOVE-Ex and SOVE-Ex). Lipid peroxidation [thiobarbituric acid-reacting substances (TBARS)] was 33% higher in FO compared with SO in the liver, but oxidative protein damage (carbonyl levels) remained similar in both liver and red gastrocnemius muscle (RG). Vitamin E supplementation, compared with FO and SO, markedly decreased liver and RG TBARS, but liver TBARS remained 32% higher in FOVE vs. SOVE. Vitamin E also markedly decreased liver and RG protein carbonyl levels, although levels in FOVE and SOVE were similar. Exercise increased liver and RG TBARS and RG protein carbonyl levels markedly, with similar levels in FOVE-Ex and SOVE-Ex. FO increased lipid peroxidation but not protein oxidation in a tissue-specific manner. Vitamin E markedly decreased lipid peroxidation and protein oxidation in both FOVE and SOVE, although liver lipid peroxidation remained higher in FOVE. Despite higher levels of hepatic lipid peroxidation at rest in FOVE compared with SOVE, liver appeared to be relatively less susceptible to exercise-induced oxidative stress in FOVE.
补充鱼油和体育锻炼可能会引发氧化应激。我们测试了8周补充α-生育酚（维生素E）和鱼油（FO）对静息状态及运动诱导的氧化应激的影响。将80只大鼠分为补充鱼油组、鱼油和维生素E组（FOVE）、大豆油组（SO）以及大豆油和维生素E组（SOVE），对于FOVE组和SOVE组，又将其分为相应的运动组（FOVE-Ex和SOVE-Ex）。肝脏中，与SO组相比，FO组的脂质过氧化[硫代巴比妥酸反应物质（TBARS）]高出33%，但肝脏和红色腓肠肌（RG）中的氧化蛋白损伤（羰基水平）保持相似。与FO组和SO组相比，补充维生素E显著降低了肝脏和RG的TBARS，但FOVE组的肝脏TBARS比SOVE组仍高出32%。维生素E也显著降低了肝脏和RG的蛋白质羰基水平，尽管FOVE组和SOVE组的水平相似。运动显著增加了肝脏和RG的TBARS以及RG的蛋白质羰基水平，FOVE-Ex组和SOVE-Ex组的水平相似。FO以组织特异性方式增加脂质过氧化但不增加蛋白质氧化。维生素E在FOVE组和SOVE组中均显著降低脂质过氧化和蛋白质氧化，尽管FOVE组的肝脏脂质过氧化仍然较高。尽管与SOVE组相比，FOVE组静息时肝脏脂质过氧化水平较高，但FOVE组的肝脏似乎对运动诱导的氧化应激相对不那么敏感。
