在尿素存在下测定人精氨酸酶的超微量方法。
Ultramicromethod for the determination of human arginase in the presence of urea.
作者信息
Nishibe H
出版信息
Clin Chim Acta. 1976 Sep 20;71(3):413-8. doi: 10.1016/0009-8981(76)90092-9.
PMID:9217
Abstract
A technique for arginase determination in body fluids in the presence of urea is described. [14C]Arginine is hydrolysed by arginase to [14C]urea and ornithine. [14C]Urea is separated with paper chromatography and measured in a liquid scintillation counter. The experimental conditions including the pH, substrate concentration, activator, solvent for chromatography, urea inhibition, and arginase in hemolysates, are discussed.
摘要
本文描述了一种在存在尿素的情况下测定体液中精氨酸酶的技术。[14C]精氨酸被精氨酸酶水解为[14C]尿素和鸟氨酸。[14C]尿素用纸上色谱法分离，并在液体闪烁计数器中进行测量。文中讨论了实验条件，包括pH值、底物浓度、激活剂、色谱溶剂、尿素抑制以及溶血产物中的精氨酸酶。
Zotero 插件