Konarska L, Tomaszewski L
Clin Chim Acta. 1986 Jan 15;154(1):7-17. doi: 10.1016/0009-8981(86)90083-5.
An accurate, precise and sensitive method has been developed for measurement of arginase activity in erythrocytes in dried blood spots. The assay is based on colorimetric measurement of ornithine produced by enzymatic hydrolysis of L-arginine. Only 10 microliter of capillary blood collected on filter paper are required. One disc of 3 mm in diameter punched from the dried blood spot is used for the arginase assay. The whole procedure is performed in one test-tube and does not need deproteinization. The second disc of the same diameter is used for hemoglobin (Hb) measurement. There was a good correlation between activities determined in dried blood spots and fresh erythrocytes of the same blood specimens taken from 101 healthy adults and 49 children (corre. coeff. 0.955 and 0.968, respectively). Arginase activity in dried blood specimens was 68.3 +/- 22.7 in adults and 62.7 +/- 15.7 U/g Hb in children. In 118 newborns, the activity was 101.9 +/- 29.2. In 1270 residents of nursing homes screened for hyperargininemia the activity was 21.5-171.2 U/g Hb. In screening for arginase deficiency, the method may be used as a simplified qualitative test without Hb assay.
已开发出一种准确、精密且灵敏的方法，用于测量干血斑中红细胞的精氨酸酶活性。该测定基于对L-精氨酸酶促水解产生的鸟氨酸的比色测定。仅需10微升采集在滤纸上的毛细血管血。从干血斑上冲下一个直径3毫米的圆片用于精氨酸酶测定。整个过程在一个试管中进行，无需脱蛋白。相同直径的第二个圆片用于血红蛋白（Hb）测量。在101名健康成年人和49名儿童的相同血液标本中，干血斑中测定的活性与新鲜红细胞中的活性之间存在良好的相关性（校正系数分别为0.955和0.968）。成年人干血标本中的精氨酸酶活性为68.3±22.7，儿童为62.7±15.7 U/g Hb。在118名新生儿中，活性为101.9±29.2。在对1270名疗养院居民进行高精氨酸血症筛查时，活性为21.5 - 171.2 U/g Hb。在精氨酸酶缺乏症筛查中，该方法可作为一种简化的定性试验，无需进行Hb测定。
